Studies of mucus in mouse stomach, small intestine, and colon. III. Gastrointestinal Muc5ac and Muc2 mucin O-glycan patterns reveal a regiospecific distribution

• The mouse intestinal mucus is mainly made up by the gel-forming Muc2 mucin and the stomach surface mucus Muc5ac, both extensively O-glycosylated. The oligosaccharide diversity provides a vast library of potential recognition sites for both commensal and pathogenic organisms. The mucin glycans are thus likely very important for the selection and maintenance of a stable intestinal flora. Here we have explored the O-glycan patterns of the mouse gastrointestinal tract mucins. The mucins from the mucus of the distal and proximal colon, ileum, jejunum, duodenum, and stomach of conventionally raised wild-type (C57BL/6) mice were separated by composite gel electrophoresis. The O-linked glycans were released by reductive elimination and structurally characterized by liquid chromatography-mass spectrometry. The mucins glycans were mostly core 2 type [Gal beta 1-3(GlcNAc beta 1-6)GalNAcol], but also core 1 (Gal beta 1-3GalNAcol). In the stomach about half of the Muc5ac mucin O-glycans were neutral and many monosulfated, but with a low grade of sialylation and fucosylation. Mouse ileum, jejunum, and duodenum had similar glycan patterns dominated by sialylated and sulfated core 2 glycans, but few fucosylated. Colon was on the other hand dominated by highly charged fucosylated glycans. The distal colon is different from the proximal colon because different biosynthetic pathways are utilized, although sialylated and sulfated glycans were highly abundant in both parts. The sulfation was higher in the distal colon, whereas sialic acid was more common in the proximal colon. Many fucosylated glycans were found in both the proximal and distal colon. Thus the mucin O-glycans vary along the mouse gastrointestinal tract.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine (hsv//eng)

MEDICIN OCH HÄLSOVETENSKAP  -- Hälsovetenskap (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Health Sciences (hsv//eng)

Nyckelord

glycomics

Mucin

Muc2

Muc5ac

mass spectrometry

oligosaccharide

glycan

BLOOD-GROUP-A

LINKED OLIGOSACCHARIDES

NIPPOSTRONGYLUS-BRASILIENSIS

HELICOBACTER-PYLORI

GUT MICROBIOTA

GLYCOSYLATION

MICE

GLYCOSYLTRANSFERASE

DIVERSITY

BACTERIA

ATES OF AMERICA

Animals

Chromatography

Liquid

Colon

metabolism

Electrophoresis

Polyacrylamide Gel

Female

Fucose

metabolism

Gastric Mucosa

metabolism

Glycosylation

Intestinal Mucosa

metabolism

Intestine

Small

metabolism

Male

Mass Spectrometry

Mice

Mice

Inbred C57BL

Mucin 5AC

metabolism

Mucin-2

metabolism

Mucus

metabolism

N-Acetylneuraminic Acid

metabolism

Proteomics

methods

Sulfates

metabolism

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