Search: WFRF:(Bruhn Sören)
> (2010-2014) >
MicroRNAs act compl...
MicroRNAs act complementarily to regulate disease-related mRNA modules in human diseases
-
- Chavali, Sreenivas (author)
- MRC Laboratory of Molecular Biology, Cambridge, United Kingdom
-
- Bruhn, Sören (author)
- Linköpings universitet,Avdelningen för kliniska vetenskaper,Hälsouniversitetet
-
- Tiemann, Katrin (author)
- Linköpings universitet,Institutionen för klinisk och experimentell medicin,Hälsouniversitetet
-
show more...
-
- Saetrom, P. (author)
- Norwegian University of Science and Technology, Trondheim, Norway
-
- Barrenäs, Fredrik, 1981 (author)
- Linköpings universitet,Avdelningen för kliniska vetenskaper,Hälsouniversitetet
-
- Saito, T. (author)
- Norwegian University of Science and Technology, Trondheim, Norway
-
- Kanduri, Kartiek, 1986 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för pediatrik,Institute of Clinical Sciences, Department of Pediatrics,University of Gothenburg, Sweden
-
- Wang, Hui (author)
- Linköpings universitet,Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för pediatrik,Institute of Clinical Sciences, Department of Pediatrics,Avdelningen för kliniska vetenskaper,Hälsouniversitetet
-
- Benson, Mikael, 1954 (author)
- Linköpings universitet,Avdelningen för kliniska vetenskaper,Hälsouniversitetet
-
show less...
-
(creator_code:org_t)
- 2013-09-23
- 2013
- English.
-
In: Rna-a Publication of the Rna Society. - : Cold Spring Harbor Laboratory. - 1355-8382 .- 1469-9001. ; 19:11, s. 1552-1562
- Related links:
-
https://gup.ub.gu.se... (primary) (free)
-
show more...
-
http://rnajournal.cs...
-
https://liu.diva-por... (primary) (Raw object)
-
https://gup.ub.gu.se...
-
https://doi.org/10.1...
-
https://urn.kb.se/re...
-
show less...
Abstract
Subject headings
Close
- MicroRNAs (miRNAs) play a key role in regulating mRNA expression, and individual miRNAs have been proposed as diagnostic and therapeutic candidates. The identification of such candidates is complicated by the involvement of multiple miRNAs and mRNAs as well as unknown disease topology of the miRNAs. Here, we investigated if disease-associated miRNAs regulate modules of disease-associated mRNAs, if those miRNAs act complementarily or synergistically, and if single or combinations of miRNAs can be targeted to alter module functions. We first analyzed publicly available miRNA and mRNA expression data for five different diseases. Integrated target prediction and network-based analysis showed that the miRNAs regulated modules of disease-relevant genes. Most of the miRNAs acted complementarily to regulate multiple mRNAs. To functionally test these findings, we repeated the analysis using our own miRNA and mRNA expression data from CD4+ T cells from patients with seasonal allergic rhinitis. This is a good model of complex diseases because of its well-defined phenotype and pathogenesis. Combined computational and functional studies confirmed that miRNAs mainly acted complementarily and that a combination of two complementary miRNAs, miR-223 and miR-139-3p, could be targeted to alter disease-relevant module functions, namely, the release of type 2 helper T-cell (Th2) cytokines. Taken together, our findings indicate that miRNAs act complementarily to regulate modules of disease-related mRNAs and can be targeted to alter disease-relevant functions.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- microRNA
- diseases
- regulation
- modules
- NETWORK-BASED ANALYSIS
- PROTEIN COMPLEXES
- EXPRESSION
- TARGETS
- PREDICTION
- DISORDERS
- RESPONSES
- CANCER
- GENES
- CELLS
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
-
Chavali, Sreeniv ...
-
Bruhn, Sören
-
Tiemann, Katrin
-
Saetrom, P.
-
Barrenäs, Fredri ...
-
Saito, T.
-
show more...
-
Kanduri, Kartiek ...
-
Wang, Hui
-
Benson, Mikael, ...
-
show less...
- About the subject
-
- NATURAL SCIENCES
-
NATURAL SCIENCES
-
and Biological Scien ...
-
and Biochemistry and ...
- Articles in the publication
-
Rna-a Publicatio ...
-
RNA
- By the university
-
University of Gothenburg
-
Linköping University