Sökning: onr:"swepub:oai:gup.ub.gu.se/186583" > Human and Methodolo...
Fältnamn | Indikatorer | Metadata |
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000 | 07686naa a2201117 4500 | |
001 | oai:gup.ub.gu.se/186583 | |
003 | SwePub | |
008 | 240910s2013 | |||||||||||000 ||eng| | |
009 | oai:lup.lub.lu.se:70b6e36a-8f83-4b01-924f-e925fbaf9634 | |
009 | oai:prod.swepub.kib.ki.se:126855312 | |
009 | oai:DiVA.org:su-92014 | |
024 | 7 | a https://gup.ub.gu.se/publication/1865832 URI |
024 | 7 | a https://doi.org/10.1089/ars.2012.47142 DOI |
024 | 7 | a https://lup.lub.lu.se/record/39269972 URI |
024 | 7 | a http://kipublications.ki.se/Default.aspx?queryparsed=id:1268553122 URI |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-920142 URI |
040 | a (SwePub)gud (SwePub)lud (SwePub)kid (SwePub)su | |
041 | a eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Barregård, Lars,d 1948u Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för samhällsmedicin och folkhälsa,Institute of Medicine, School of Public Health and Community Medicine4 aut0 (Swepub:gu)xbarrl |
245 | 1 0 | a Human and Methodological Sources of Variability in the Measurement of Urinary 8-Oxo-7,8-dihydro-2 '-deoxyguanosine |
264 | 1 | b Mary Ann Liebert Inc,c 2013 |
520 | a Aims: Urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) is a widely used biomarker of oxidative stress. However, variability between chromatographic and ELISA methods hampers interpretation of data, and this variability may increase should urine composition differ between individuals, leading to assay interference. Furthermore, optimal urine sampling conditions are not well defined. We performed inter-laboratory comparisons of 8-oxodG measurement between mass spectrometric-, electrochemical- and ELISA-based methods, using common within-technique calibrants to analyze 8-oxodG-spiked phosphate-buffered saline and urine samples. We also investigated human subject- and sample collection-related variables, as potential sources of variability. Results: Chromatographic assays showed high agreement across urines from different subjects, whereas ELISAs showed far more inter-laboratory variation and generally overestimated levels, compared to the chromatographic assays. Excretion rates in timed 'spot' samples showed strong correlations with 24 h excretion (the 'gold' standard) of urinary 8-oxodG (r(p) 0.67-0.90), although the associations were weaker for 8-oxodG adjusted for creatinine or specific gravity (SG). The within-individual excretion of 8-oxodG varied only moderately between days (CV 17% for 24 h excretion and 20% for first void, creatinine-corrected samples). Innovation: This is the first comprehensive study of both human and methodological factors influencing 8-oxodG measurement, providing key information for future studies with this important biomarker. Conclusion: ELISA variability is greater than chromatographic assay variability, and cannot determine absolute levels of 8-oxodG. Use of standardized calibrants greatly improves intra-technique agreement and, for the chromatographic assays, importantly allows integration of results for pooled analyses. If 24 h samples are not feasible, creatinine- or SG-adjusted first morning samples are recommended. | |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Endokrinologi och diabetes0 (SwePub)302052 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Endocrinology and Diabetes0 (SwePub)302052 hsv//eng |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Cell- och molekylärbiologi0 (SwePub)301082 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Cell and Molecular Biology0 (SwePub)301082 hsv//eng |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinsk bioteknologix Biomedicinsk laboratorievetenskap/teknologi0 (SwePub)304022 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Medical Biotechnologyx Biomedical Laboratory Science/Technology0 (SwePub)304022 hsv//eng |
650 | 7 | a NATURVETENSKAPx Biologix Biokemi och molekylärbiologi0 (SwePub)106022 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Biochemistry and Molecular Biology0 (SwePub)106022 hsv//eng |
653 | a performance liquid-chromatography | |
653 | a tandem mass-spectrometry | |
653 | a oxidative | |
653 | a stress | |
653 | a automated-method | |
653 | a in-vivo | |
653 | a dna | |
653 | a excretion | |
653 | a damage | |
653 | a 8-hydroxy-2'-deoxyguanosine | |
653 | a 8-hydroxydeoxyguanosine | |
700 | 1 | a Moller, P.4 aut |
700 | 1 | a Henriksen, T.4 aut |
700 | 1 | a Mistry, V.4 aut |
700 | 1 | a Koppen, G.4 aut |
700 | 1 | a Rossner, P.4 aut |
700 | 1 | a Sram, R. J.4 aut |
700 | 1 | a Weimann, A.4 aut |
700 | 1 | a Poulsen, H. E.4 aut |
700 | 1 | a Nataf, R.4 aut |
700 | 1 | a Andreoli, R.4 aut |
700 | 1 | a Manini, P.4 aut |
700 | 1 | a Marczylo, T.4 aut |
700 | 1 | a Lam, P.4 aut |
700 | 1 | a Evans, M. D.4 aut |
700 | 1 | a Kasai, H.4 aut |
700 | 1 | a Kawai, K.u Karolinska Institutet4 aut |
700 | 1 | a Li, Y. S.4 aut |
700 | 1 | a Sakai, K.4 aut |
700 | 1 | a Singh, R.4 aut |
700 | 1 | a Teichert, F.4 aut |
700 | 1 | a Farmer, P. B.4 aut |
700 | 1 | a Rozalski, R.4 aut |
700 | 1 | a Gackowski, D.4 aut |
700 | 1 | a Siomek, A.4 aut |
700 | 1 | a Saez, G. T.4 aut |
700 | 1 | a Cerda, C.4 aut |
700 | 1 | a Broberg Palmgren, Karinu Lund University,Lunds universitet,Avdelningen för arbets- och miljömedicin,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Occupational and Environmental Medicine, Lund University,Department of Laboratory Medicine,Faculty of Medicine4 aut0 (Swepub:lu)kgen-kbr |
700 | 1 | a Lindh, Christianu Lund University,Lunds universitet,Avdelningen för arbets- och miljömedicin,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Occupational and Environmental Medicine, Lund University,Department of Laboratory Medicine,Faculty of Medicine4 aut0 (Swepub:lu)ymed-cli |
700 | 1 | a Hossain, Bakhtiaru Lund University,Lunds universitet,Avdelningen för arbets- och miljömedicin,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Occupational and Environmental Medicine, Lund University,Department of Laboratory Medicine,Faculty of Medicine4 aut0 (Swepub:lu)med-bkh |
700 | 1 | a Haghdoost, Siamaku Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut4 aut0 (Swepub:su)shagh |
700 | 1 | a Hu, C. W.4 aut |
700 | 1 | a Chao, M. R.4 aut |
700 | 1 | a Wu, K. Y.4 aut |
700 | 1 | a Orhan, H.4 aut |
700 | 1 | a Senduran, N.4 aut |
700 | 1 | a Smith, R. J.4 aut |
700 | 1 | a Santella, R. M.4 aut |
700 | 1 | a Su, Y. L.4 aut |
700 | 1 | a Cortez, C.4 aut |
700 | 1 | a Yeh, S.4 aut |
700 | 1 | a Olinski, R.4 aut |
700 | 1 | a Loft, S.4 aut |
700 | 1 | a Cooke, M. S.4 aut |
710 | 2 | a Göteborgs universitetb Institutionen för medicin, avdelningen för samhällsmedicin och folkhälsa4 org |
773 | 0 | t Antioxidants and Redox Signalingd : Mary Ann Liebert Incg 18:18, s. 2377-2391q 18:18<2377-2391x 1523-0864x 1557-7716 |
856 | 4 | u https://europepmc.org/articles/pmc3671631?pdf=render |
856 | 4 | u http://dx.doi.org/10.1089/ars.2012.4714y FULLTEXT |
856 | 4 8 | u https://gup.ub.gu.se/publication/186583 |
856 | 4 8 | u https://doi.org/10.1089/ars.2012.4714 |
856 | 4 8 | u https://lup.lub.lu.se/record/3926997 |
856 | 4 8 | u http://kipublications.ki.se/Default.aspx?queryparsed=id:126855312 |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-92014 |
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