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Simultaneous enanti...
Simultaneous enantiospecific separation and quantitation of mephenytoin and its metabolites nirvanol and 4'-hydroxymephenytoin in human plasma by liquid chromatography.
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- Jansson, Britt (författare)
- Uppsala universitet,Avdelningen för farmakokinetik och läkemedelsterapi
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- Simonsson, Ulrika S H (författare)
- Uppsala universitet,Avdelningen för farmakokinetik och läkemedelsterapi
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- Ashton, Michael, 1955 (författare)
- Uppsala universitet,Gothenburg University,Göteborgs universitet,Institutionen för fysiologi och farmakologi, Avdelningen för farmakologi,Institute of Physiology and Pharmacology, Dept of Pharmacology,Avdelningen för farmakokinetik och läkemedelsterapi
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(creator_code:org_t)
- 2003
- 2003
- Engelska.
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Ingår i: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. - 1570-0232 .- 1873-376X. ; 791:1-2, s. 179-91
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https://gup.ub.gu.se...
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Abstract
Ämnesord
Stäng
- A high-performance liquid chromatographic method for the enantiospecific quantitation of S- and R-mephenytoin and its metabolites S- and R-nirvanol and S- and R-4'-hydroxymephenytoin in plasma is described. The compounds were separated using a reversed-phase C(2) column in tandem with a chiral alpha(1)-acid glycoprotein column and were detected using ultraviolet detection at 205 nm. The lower limit of quantification was 10 ng/ml for all compounds using 0.5 ml human plasma (intra-day coefficient of variation <13%, accuracy <+/-20%). The method was validated for human plasma in the concentration range 10-2000 ng/ml for each of the six compounds. The method allows for the simultaneous characterisation of the metabolic capacity of two human drug-metabolising enzymes, CYP2C19 and CYP2B6, and may be used when investigating polymorphisms or changes in activity of these two enzymes.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Farmaceutiska vetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Pharmaceutical Sciences (hsv//eng)
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
Nyckelord
- Aryl Hydrocarbon Hydroxylases
- metabolism
- Chromatography
- High Pressure Liquid
- Humans
- Mephenytoin
- analogs & derivatives
- blood
- isolation & purification
- Mixed Function Oxygenases
- metabolism
- Oxidoreductases
- N-Demethylating
- metabolism
- Reference Standards
- Reproducibility of Results
- Sensitivity and Specificity
- Spectrophotometry
- Ultraviolet
- Stereoisomerism
- mephenytoin
- PHARMACY
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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