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  • Ahemaiti, Aikeremu,1984Chalmers tekniska högskola,Chalmers University of Technology (author)

A multifunctional pipette for localized drug administration to brain slices

  • Article/chapterEnglish2013

Publisher, publication year, extent ...

  • Elsevier BV,2013

Numbers

  • LIBRIS-ID:oai:gup.ub.gu.se/189124
  • https://gup.ub.gu.se/publication/189124URI
  • https://doi.org/10.1016/j.jneumeth.2013.08.012DOI
  • https://research.chalmers.se/publication/189124URI
  • http://kipublications.ki.se/Default.aspx?queryparsed=id:127719686URI

Supplementary language notes

  • Language:English

Part of subdatabase

Classification

  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • We have developed a superfusion method utilizing an open-volume microfluidic device for administration of pharmacologically active substances to selected areas in brain slices with high spatio-temporal resolution. The method consists of a hydrodynamically confined flow of the active chemical compound, which locally stimulates neurons in brain slices, applied in conjunction with electrophysiological recording techniques to analyze the response. The microfluidic device, which is a novel free-standing multifunctional pipette, allows diverse superfusion experiments, such as testing the effects of different concentrations of drugs or drug candidates on neurons in different cell layers with high positional accuracy, affecting only a small number of cells. We demonstrate herein the use of the method with electrophysiological recordings of pyramidal cells in hippocampal and prefrontal cortex brain slices from rats, determine the dependence of electric responses on the distance of the superfusion device from the recording site, document a multifold gain in solution exchange time as compared to whole slice perfusion, and show that the device is able to store and deliver up to four solutions in a series. Localized solution delivery by means of open-volume microfluidic technology also reduces reagent consumption and tissue culture expenses significantly, while allowing more data to be collected from a single tissue slice, thus reducing the number of laboratory animals to be sacrificed for a study. (C) 2013 Elsevier B.V. All rights reserved.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

  • Ainla, Alar,1982Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)ainla (author)
  • Jeffries, Gavin,1980Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)jeffries (author)
  • Wigström, Holger,1946Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för fysiologi,Institute of Neuroscience and Physiology, Department of Physiology,University of Gothenburg(Swepub:gu)xwigho (author)
  • Orwar, Owe,1964Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)orwar (author)
  • Jesorka, Aldo,1967Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)aldo (author)
  • Jardemark, Kent Eric,1962Karolinska Institutet(Swepub:cth)kenter (author)
  • Chalmers tekniska högskolaInstitutionen för neurovetenskap och fysiologi, sektionen för fysiologi (creator_code:org_t)

Related titles

  • In:Journal of Neuroscience Methods: Elsevier BV219:2, s. 292-2960165-02701872-678X

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