Two modes of exocytosis in an artificial cell

Mellander, Lisa J.Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,University of Gothenburg (författare)

The details of exocytosis, the vital cell process of neuronal communication, are still under debate with two generally accepted scenarios. The first mode of release involves secretory vesicles distending into the cell membrane to release the complete vesicle contents. The second involves partial release of the vesicle content through an intermittent fusion pore, or an opened or partially distended fusion pore. Here we show that both full and partial release can be mimicked with a single large-scale cell model for exocytosis composed of material from blebbing cell plasma membrane. The apparent switching mechanism for determining the mode of release is demonstrated to be related to membrane tension that can be differentially induced during artificial exocytosis. These results suggest that the partial distension mode might correspond to an extended kiss-and-run mechanism of release from secretory cells, which has been proposed as a major pathway of exocytosis in neurons and neuroendocrine cells.

Kurczy, Michael,1980Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)kurczy (författare)
Najafinobar, Neda,1985Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)nobar (författare)
Dunevall, Johan,1984Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)dunevall (författare)
Ewing, Andrew G,1957Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,Chalmers tekniska högskola,Chalmers University of Technology,University of Gothenburg(Swepub:cth)andrewe (författare)
Cans, Ann-Sofie,1971Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)cans (författare)
Göteborgs universitetInstitutionen för kemi och molekylärbiologi (creator_code:org_t)

Av författaren/redakt...: Mellander, Lisa ...; Kurczy, Michael, ...; Najafinobar, Ned ...; Dunevall, Johan, ...; Ewing, Andrew G, ...; Cans, Ann-Sofie, ...

Om ämnet

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.