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Real-time PCR thres...
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Elfving, KristinaGothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för pediatrik,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Institute of Clinical Sciences, Department of Pediatrics,Institute of Biomedicine, Department of Infectious Medicine
(author)
Real-time PCR threshold cycle (Ct) cut-offs help to identify agents causing acute childhood diarrhea in Zanzibar.
- Article/chapterEnglish2014
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LIBRIS-ID:oai:gup.ub.gu.se/194115
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https://gup.ub.gu.se/publication/194115URI
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https://doi.org/10.1128/JCM.02697-13DOI
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http://kipublications.ki.se/Default.aspx?queryparsed=id:128436294URI
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Subject category:art swepub-publicationtype
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Molecular assays might improve identification of causes of acute diarrheal disease, but may lead to more frequent detection of asymptomatic infections. In the present study real-time PCR targeting 14 pathogens was applied on rectal swabs from 330 children aged 2-59 months in Zanzibar, 165 with acute diarrhea and 165 asymptomatic controls. At least one pathogen was detected in 94% of patients and 84% of controls, with higher rates in patients for norovirus genogroup II (20% vs. 2.4%, p<0.0001), rotavirus (10% vs. 1.8%, p=0.003) and Cryptosporidium (30% vs. 11%, p<0.0001). Detection rates did not differ significantly for enterotoxigenic Escherichia coli (ETEC)-estA (33% vs. 24%), ETEC-eltB (44% vs. 46%), Shigella (35% vs. 33%), and Campylobacter (35% vs. 33%), but for these agents Ct (threshold cycle) values were lower (pathogen loads were higher) in sick children than in controls. In multivariate analysis, Ct values for norovirus genogroup II, rotavirus, Cryptosporidium, ETEC-estA and Shigella were independently associated with diarrhea. We conclude that this real-time PCR allows convenient detection of essentially all diarrheagenic agents, and provides Ct values that may be critical for interpretation of results for pathogens with similar detection rates in patients and controls. The results indicate that assessment of pathogen load may improve identification of agents causing gastroenteritis in children.
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Andersson, MariaGothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Institute of Biomedicine, Department of Infectious Medicine
(author)
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Msellem, Mwinyi I
(author)
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Welinder-Olsson, Christina,1959Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Institute of Biomedicine, Department of Infectious Medicine(Swepub:gu)xwelch
(author)
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Petzold, Max,1973Gothenburg University,Göteborgs universitet,Akademistatistik,Centre for Applied Biostatistics(Swepub:gu)xpemax
(author)
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Björkman, AndersKarolinska Institutet
(author)
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Trollfors, Birger,1947Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för pediatrik,Institute of Clinical Sciences, Department of Pediatrics(Swepub:gu)xtrobi
(author)
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Mårtensson, AndreasKarolinska Institutet
(author)
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Lindh, Magnus,1960Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Institute of Biomedicine, Department of Infectious Medicine(Swepub:gu)xlmagr
(author)
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Göteborgs universitetInstitutionen för kliniska vetenskaper, Avdelningen för pediatrik
(creator_code:org_t)
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In:Journal of clinical microbiology52:3, s. 916-9231098-660X
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