Cytokine release during long-term extracorporeal circulation in an experimental model.

• The objective of this study was to determine the degree of leukocyte activation, as measured by cytokine release, in circulating blood during experimental extracorporeal circulation. Complete in vitro extracorporeal membrane oxygenation (ECMO) circuits were used, and 9 experiments were performed. Whole blood stored at 37 degrees C was used as the control. Blood samples were withdrawn before the start of perfusion and at 24 h of perfusion. Statistically significant releases of interleukin (IL)-1beta, IL-8, and IL-1 receptor antagonist were observed in the perfusion circuits compared to both the control blood and baseline values. Also, increases in plasma tumor necrosis factor (TNF)alpha and IL-6 were seen after 24 h of perfusion although these changes did not reach statistical significance. These results indicate that extracorporeal circulation induced leukocyte activation and cytokine release. These reactions might, as an additional trauma, deteriorate the situation in an already severely ill patient. A search for methods to counteract this untoward activation seems warranted.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine (hsv//eng)

Nyckelord

Adult

Anticoagulants

blood

Blood Cell Count

Chemotaxis

Leukocyte

physiology

Critical Illness

Cytokines

blood

Equipment Design

Extracorporeal Circulation

instrumentation

methods

Hemoglobins

analysis

Heparin

blood

Humans

Interleukin-1

blood

Interleukin-6

blood

Interleukin-8

blood

Leukocyte Count

Leukocytes

physiology

Neutrophils

cytology

Oxygenators

Platelet Count

Receptors

Interleukin-1

antagonists & inhibitors

Tumor Necrosis Factor-alpha

analysis

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art (ämneskategori)