Biotinylated platelets have an impaired response to agonists as evidenced by in vitro platelet aggregation tests.

• Biotinylation of platelets, using a water soluble biotin analogue which reacts with primary amines, has been proposed to be a reliable technique for study of in vivo survival of platelets and their subpopulations. The information about the influence of this technique on platelet function has been limited. In the present work we studied the effect of in vitro biotinylation on platelet function and activation. Washed human platelets, at a concentration of 1 x 10(9)/L, were biotinylated with five different concentrations of sulfo-NHS-biotin or NHS-LC-biotin, ranging from 0 to 5 mM. The degree of platelet activation during and after biotinylation was monitored by measuring the externalization of P-selectin, and the platelet function was evaluated by aggregometry. It was observed that biotinylated platelets, in a dose dependent manner, displayed an impaired aggregation response. A slight increase in platelet membrane P-selectin occurred during the labelling procedure.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine (hsv//eng)

Nyckelord

Adenosine Diphosphate

pharmacology

Adrenergic Agonists

pharmacology

Biotin

analogs & derivatives

pharmacology

Biotinylation

Blood Platelets

physiology

Blotting

Western

Dose-Response Relationship

Drug

Epinephrine

pharmacology

Flow Cytometry

Hemostatics

pharmacology

Humans

P-Selectin

biosynthesis

drug effects

Platelet Aggregation

drug effects

Stimulation

Chemical

Succinimides

pharmacology

Thrombin

pharmacology

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