Detection of IL-17A-producing peripheral blood monocytes in Langerhans cell histiocytosis patients

• Langerhans cell histiocytosis (LCH) is a rare disease of unknown cause with manifestations ranging from isolated granulomatous lesions to life-threatening multi-system organ involvement. This disorder is further characterized by infiltration of immune cells in affected tissues and an association with interleukin (IL)-17A has been reported. Here, we investigated the presence of IL-17A-producing cells among peripheral blood mononuclear cells isolated from LCH patients and observed a high percentage of IL-17A(+) monocytes in peripheral blood of LCH patients compared to controls. The IL-17A(+) monocytes were also positive for the transcription factor retinoic acid orphan receptor (ROR) gamma t and showed increased mRNA levels for both IL-17A and ROR gamma t. Notably, IL-17A was produced by all monocyte subsets and the expression level was positively associated with LCH disease activity. These data support a role for monocytes in the pathogenesis of LCH. Future therapeutic approaches may consider identification of patients who may benefit from IL-17A-targeted interventions. (C) 2014 Elsevier Inc. All rights reserved.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Immunology in the medical area (hsv//eng)

Nyckelord

Monocytes

Langerhans cell histiocytosis

Interleukin-17A

Retinoic acid orphan receptor gamma t

DENDRITIC CELLS

RHEUMATOID-ARTHRITIS

MULTIPLE-MYELOMA

IL-17A

RECEPTOR

CYTOKINE GENES

RETINOIC ACID

SERUM-LEVELS

T-CELLS

SYSTEM

LYMPHOCYTES

Immunology

Monocytes

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)