Hydrogen peroxide contributes to the ultraviolet-B (280-315 nm) induced oxidative stress of plant leaves through multiple pathways

• Solar UV-B (280-315 nm) radiation is a developmental signal in plants but may also cause oxidative stress when combined with other environmental factors. Using computer modeling and in solution experiments we show that UV-B is capable of photosensitizing hydroxyl radical production from hydrogen peroxide. We present evidence that the oxidative effect of UV-B in leaves is at least twofold: (i) it increases cellular hydrogen peroxide concentrations, to a larger extent in pyridoxine antioxidant mutant pdx1.3-1 Arabidopsis and; (ii) is capable of a partial photo-conversion of both 'natural' and 'extra' hydrogen peroxide to hydroxyl radicals. As stress conditions other than UV can increase cellular hydrogen peroxide levels, synergistic deleterious effects of various stresses may be expected already under ambient solar UV-B. (C) 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Ämnesord

NATURVETENSKAP  -- Biologi -- Cellbiologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Cell Biology (hsv//eng)

NATURVETENSKAP  -- Biologi -- Biofysik (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Biophysics (hsv//eng)

NATURVETENSKAP  -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)

Nyckelord

Arabidopsis

Computer modeling

Hydrogen peroxide

Hydroxyl radical

Leaf

Oxygen radicals

Plant

ELECTRON-SPIN RESONANCE

UV-B

REACTIVE OXYGEN

THYLAKOID MEMBRANES

PHOTOSYSTEM-II

RADIATION

PHOTOLYSIS

RADICALS

PHOTOSYNTHESIS

CHLOROPLASTS

Biochemistry & Molecular Biology

Biophysics

Cell Biology

Arabidopsis; Computer modeling; Hydrogen peroxide; Hydroxyl radical; Leaf; Oxygen radicals; Photosynthesis; Plant; Terephthalic acid; UV photobiology

Biokemi

Publikations- och innehållstyp

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