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FKBP5 expression in...
FKBP5 expression in human adipose tissue increases following dexamethasone exposure and is associated with insulin resistance
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- Pereira, Maria J, 1981- (författare)
- Uppsala universitet,Klinisk diabetologi och metabolism
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Palming, J. (författare)
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Svensson, M.K. (författare)
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Rizell, M. (författare)
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Dalenbäck, J.A. (författare)
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Hammar, M. (författare)
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- Fall, Tove (författare)
- Uppsala universitet,Molekylär epidemiologi
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- Sidibeh, Cherno O (författare)
- Uppsala universitet,Klinisk diabetologi och metabolism
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- Svensson, Per-Arne, 1969 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för molekylär och klinisk medicin,Institute of Medicine, Department of Molecular and Clinical Medicine
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- Eriksson, Jan W (författare)
- Uppsala universitet,Klinisk diabetologi och metabolism
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(creator_code:org_t)
- Elsevier BV, 2014
- 2014
- Engelska.
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Ingår i: Metabolism: Clinical and Experimental. - : Elsevier BV. - 0026-0495 .- 1532-8600. ; 63:9, s. 1198-1208
- Relaterad länk:
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- Objective To study effects of dexamethasone on gene expression in human adipose tissue aiming to identify potential novel mechanisms for glucocorticoid-induced insulin resistance. Materials/methods Subcutaneous and omental adipose tissue, obtained from non-diabetic donors (10 M/15 F; age: 28-60 years; BMI: 20.7-30.6 kg/m2), was incubated with or without dexamethasone (0.003-3 μmol/L) for 24 h. Gene expression was assessed by microarray and real time-PCR and protein expression by immunoblotting. Results FKBP5 (FK506-binding protein 5) and CNR1 (cannabinoid receptor 1) were the most responsive genes to dexamethasone in both subcutaneous and omental adipose tissue (~ 7-fold). Dexamethasone increased FKBP5 gene and protein expression in a dose-dependent manner in both depots. The gene product, FKBP51 protein, was 10-fold higher in the omental than in the subcutaneous depot, whereas the mRNA levels were similar. Higher FKBP5 gene expression in omental adipose tissue was associated with reduced insulin effects on glucose uptake in both depots. Furthermore, FKBP5 gene expression in subcutaneous adipose tissue was positively correlated with serum insulin, HOMA-IR and subcutaneous adipocyte diameter and negatively with plasma HDL-cholesterol. FKBP5 SNPs were found to be associated with type 2 diabetes and diabetes-related phenotypes in large population-based samples. Conclusions Dexamethasone exposure promotes expression of FKBP5 in adipose tissue, a gene that may be implicated in glucocorticoid-induced insulin resistance. © 2014 Elsevier Inc.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Endokrinologi och diabetes (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Endocrinology and Diabetes (hsv//eng)
Nyckelord
- Adipose tissue
- FKBP5
- Gene expression
- Glucocorticoids
- Insulin resistance
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Pereira, Maria J ...
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Palming, J.
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Svensson, M.K.
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Rizell, M.
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Dalenbäck, J.A.
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Hammar, M.
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visa fler...
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Fall, Tove
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Sidibeh, Cherno ...
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Svensson, Per-Ar ...
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Eriksson, Jan W
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visa färre...
- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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och Endokrinologi oc ...
- Artiklar i publikationen
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Metabolism: Clin ...
- Av lärosätet
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Göteborgs universitet
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Uppsala universitet