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Blood group A glyco...
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Holgersson, J
(författare)
Blood group A glycolipid antigen biosynthesis: discrimination between biosynthesized and enzyme preparation derived blood group A antigen by mass spectrometry.
- Artikel/kapitelEngelska1990
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LIBRIS-ID:oai:gup.ub.gu.se/211807
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https://gup.ub.gu.se/publication/211807URI
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A monofucosyl type 1 chain blood group A hexaglycosylceramide was biosynthesized in solution using the type 1 chain blood group H pentaglycosylceramide as precursor, a crude microsomal fraction prepared from the mucosa scraping of a blood group A pig small intestine as enzyme source, and uridine diphosphate-N-acetyl-(1-14C)galactosamine as sugar donor. The radioactive product was enriched using reversed-phase column chromatography and silica gel HPLC. The peak, as detected by a beta-flow scintillation counter, was collected, permethylated, and analyzed by mass spectrometry. Carbohydrate sequence ions were found, indicating the presence of both the biosynthesized and a native, non-14C-containing blood group A hexaglycosylceramide. The blood group A pig small intestinal mucosa used as the enzyme source contain blood group A hexaglycosylceramide as the predominant glycolipid. Therefore, it is concluded that the nonradioactive blood group A hexaglycosylceramide found after the biosynthesis is derived from the enzyme preparation.
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Jacobsson, A
(författare)
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Breimer, Michael,1951Gothenburg University,Göteborgs universitet,Medicinska institutionen,Department medicine(Swepub:gu)xbremi
(författare)
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Samuelsson, B E
(författare)
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Göteborgs universitetMedicinska institutionen
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Analytical biochemistry184:1, s. 145-500003-2697
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