The effect of exercise on angiogenic factors in the healthy mouse heart: A short report

• Background: Exercise increases blood levels of crucial angiogenic factors and endothelial progenitor cells (EPCs). Hypoxia inducible factor-1 (HIF-1a) and vascular endothelial growth factor (VEGF) are also increased in skeletal muscle in response to exercise. In the healthy heart, voluntary exercise is not expected to cause local hypoxia. We studied how voluntary exercise affects cardiac expression of HIF-1a, VEGF and stromal derived factor-1 (SDF-1), as well as EPC levels in heart and skeletal muscle. Method: Thirty-two NMRI mice were randomized to exercise in running wheels (EX) or regular activity (SED). HIF-1a, VEGF and SDF-1 mRNA levels were analyzed by reverse transcription polymerase chain reaction (RT-PCR) and EPC levels in heart and hind limb were quantified by FACS after 7 and 14 days. Results: There was no significant difference in cardiac expression of HIF-1a, VEGF or SDF-1 between EX and SED. Cardiac EPC levels were not affected by exercise, while skeletal EPC level was more than doubled. Conclusion: Voluntary exercise does not seem to induce cardiac hypoxia or stimulate the angiogenic system. In the healthy normoxic heart, there is a limited need of supporting blood supply, which might explain these findings.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Hälsovetenskap (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Health Sciences (hsv//eng)

Nyckelord

Endothelial progenitor cells

Exercise

Heart

angiogenic factor

hypoxia inducible factor 1alpha

messenger RNA

stromal cell derived factor 1

vasculotropin

angiogenesis

animal experiment

animal tissue

article

cardiovascular effect

cardiovascular risk

controlled study

coronary artery blood flow

endothelial progenitor cell

female

fluorescence activated cell sorting

heart muscle

hindlimb

hypoxia

mouse

muscle growth

NMRI mouse

nonhuman

protein expression

reverse transcription polymerase chain reaction

sedentary lifestyle

sitting

treadmill exercise

Medicin/Teknik

Publikations- och innehållstyp

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art (ämneskategori)