Sökning: WFRF:(Wigström Holger 1946)
> (2015-2019) >
Spatial characteriz...
Spatial characterization of a multifunctional pipette for drug delivery in hippocampal brain slices
-
- Ahemaiti, Aikeremu, 1984 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
-
- Wigström, Holger, 1946 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för fysiologi,Institute of Neuroscience and Physiology, Department of Physiology,University of Gothenburg
-
- Ainla, Alar, 1982 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
-
visa fler...
-
- Jeffries, Gavin, 1980 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
-
- Orwar, Owe, 1964 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
-
- Jesorka, Aldo, 1967 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
-
- Jardemark, Kent Eric, 1962 (författare)
- Karolinska Institutet
-
visa färre...
-
(creator_code:org_t)
- Elsevier BV, 2015
- 2015
- Engelska.
-
Ingår i: Journal of Neuroscience Methods. - : Elsevier BV. - 0165-0270 .- 1872-678X. ; 241, s. 132-136
- Relaterad länk:
-
https://europepmc.or...
-
visa fler...
-
https://gup.ub.gu.se...
-
https://doi.org/10.1...
-
https://research.cha...
-
http://kipublication...
-
visa färre...
Abstract
Ämnesord
Stäng
- Background: Among the various fluidic control technologies, microfluidic devices are becoming powerful tools for pharmacological studies using brain slices, since these devices overcome traditional limitations of conventional submerged slice chambers, leading to better spatiotemporal control over delivery of drugs to specific regions in the slices. However, microfluidic devices are not yet fully optimized for such studies. New method: We have recently developed a multifunctional pipette (MFP), a free standing hydrodynamically confined microfluidic device, which provides improved spatiotemporal control over drug delivery to biological tissues. Results: We demonstrate herein the ability of the MFP to selectively perfuse one dendritic layer in the CA1 region of hippocampus with CNQX, an AMPA receptor antagonist, while not affecting the other layers in this region. Our experiments also illustrate the essential role of hydrodynamic confinement in sharpening the spatial selectivity in brain slice experiments. Concentration-response measurements revealed that the ability of the MFP to control local drug concentration is comparable with that of whole slice perfusion, while in comparison the required amounts of active compounds can be reduced by several orders of magnitude. Comparison with existing method: The multifunctional pipette is applied with an angle, which, compared to other hydrodynamically confined microfluidic devices, provides more accessible space for other probing and imaging techniques. Conclusions: Using the MFP it will be possible to study selected regions of brain slices, integrated with various imaging and probing techniques, without affecting the other parts of the slices.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Neurologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Neurology (hsv//eng)
- NATURVETENSKAP -- Kemi -- Fysikalisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Physical Chemistry (hsv//eng)
Nyckelord
- Microfluidic technology
- Perfusion
- Multifunctional pipette
- Brain slices
- Hippocampus
- Glutamate
- Electrophysiological techniques
- Brain slices
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Ahemaiti, Aikere ...
-
Wigström, Holger ...
-
Ainla, Alar, 198 ...
-
Jeffries, Gavin, ...
-
Orwar, Owe, 1964
-
Jesorka, Aldo, 1 ...
-
visa fler...
-
Jardemark, Kent ...
-
visa färre...
- Om ämnet
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Klinisk medicin
-
och Neurologi
-
- NATURVETENSKAP
-
NATURVETENSKAP
-
och Kemi
-
och Fysikalisk kemi
- Artiklar i publikationen
-
Journal of Neuro ...
- Av lärosätet
-
Göteborgs universitet
-
Chalmers tekniska högskola
-
Karolinska Institutet