Dysfunction of Platelet-derived Growth Factor Receptor a (PDGFR alpha) Represses the Production of Oligodendrocytes from Arylsulfatase A-deficient Multipotential Neural Precursor Cells

The membrane-bound receptor for platelet-derived growth factor A (PDGFR alpha) is crucial for controlling the production of oligodendrocytes (OLs) for myelination, but regulation of its activity during OL differentiation is largely unknown. We have examined the effect of increased sulfated content of galactosylceramides (sulfatides) on the regulation of PDGTR alpha in multipotential neural precursors (NPs) that are deficient in arylsulfatase A (ASA) activity. This enzyme is responsible for the lysosomal hydrolysis of sulfatides. We show that sulfatide accumulation significantly impacts the formation of OLs via deregulation of PDGFR alpha function. PDGFR alpha is less associated with detergent-resistant membranes in ASA-deficient cells and showed a significant decrease in AKT phosphorylation Rescue experiments with ASA showed a normalization of the ratio of long versus short sulfatides, restored PDGTRa levels, corrected its localization to detergent-resistant membranes, increased AKT phosphorylation, and normalized the production of OLs in ASA-deficient NPs. Moreover, our studies identified a novel mechanism that regulates the secretion of PDGFR alpha in NPs, in glial cells, and in the brain cortex via exosomal shedding. Our study provides a first step in understanding the role of sulfatides in regulating PDGFR alpha levels in OLs and its impact in myelination.

NATURVETENSKAP Biologi Biokemi och molekylärbiologi hsv//swe
NATURAL SCIENCES Biological Sciences Biochemistry and Molecular Biology hsv//eng
CENTRAL-NERVOUS-SYSTEM
SPINAL-CORD OLIGODENDROCYTES
METACHROMATIC
LEUKODYSTROPHY
PROGENITOR CELLS
MYELIN MEMBRANE
SONIC HEDGEHOG
LIPID
RAFTS
INTRACELLULAR TRAFFICKING
MULTIVESICULAR ENDOSOMES
RETICULOCYTE
MATURATION
Biochemistry & Molecular Biology

Moyano, A. L. (author)
Lopez-Rosas, A. (author)
Marottoli, F. M. (author)
Li, G. N. (author)
Hu, C. Q. (author)
van Breemen, R. (author)
Månsson, Jan-Eric,1946Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin,Institute of Biomedicine, Department of Clinical Chemistry and Transfusion Medicine(Swepub:gu)xmanja (author)
Givogri, M. I. (author)
Göteborgs universitetInstitutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin (creator_code:org_t)

By the author/editor: Pituch, K. C.; Moyano, A. L.; Lopez-Rosas, A.; Marottoli, F. M.; Li, G. N.; Hu, C. Q.; show more...; van Breemen, R.; Månsson, Jan-Eri ...; Givogri, M. I.; show less...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Biochemistry and ...

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