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A Strategy for O-Gl...
A Strategy for O-Glycoproteomics of Enveloped Viruses-the O-Glycoproteome of Herpes Simplex Virus Type 1
- Article/chapterEnglish2015
Publisher, publication year, extent ...
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2015-04-01
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Public Library of Science (PLoS),2015
Numbers
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LIBRIS-ID:oai:gup.ub.gu.se/218709
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https://gup.ub.gu.se/publication/218709URI
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https://doi.org/10.1371/journal.ppat.1004784DOI
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Glycosylation of viral envelope proteins is important for infectivity and interaction with host immunity, however, our current knowledge of the functions of glycosylation is largely limited to N-glycosylation because it is difficult to predict and identify site-specific O-glycosylation. Here, we present a novel proteome-wide discovery strategy for O-glycosylation sites on viral envelope proteins using herpes simplex virus type 1 (HSV-1) as a model. We identified 74 O-linked glycosylation sites on 8 out of the 12 HSV-1 envelope proteins. Two of the identified glycosites found in glycoprotein B were previously implicated in virus attachment to immune cells. We show that HSV-1 infection distorts the secretory pathway and that infected cells accumulate glycoproteins with truncated O-glycans, nonetheless retaining the ability to elongate most of the surface glycans. With the use of precise gene editing, we further demonstrate that elongated O-glycans are essential for HSV-1 in human HaCaT keratinocytes, where HSV-1 produced markedly lower viral titers in HaCaT with abrogated O-glycans compared to the isogenic counterpart with normal O-glycans. The roles of O-linked glycosylation for viral entry, formation, secretion, and immune recognition are poorly understood, and the O-glycoproteomics strategy presented here now opens for unbiased discovery on all enveloped viruses.
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Nordén, Rickard,1977Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Institute of Biomedicine, Department of Infectious Medicine(Swepub:gu)xnorri
(author)
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Joshi, H. J.
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Dabelsteen, S.
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Nyström, Kristina,1977Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Institute of Biomedicine, Department of Infectious Medicine(Swepub:gu)xnystk
(author)
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Vakhrushev, S. Y.
(author)
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Olofsson, Sigvard,1948Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Institute of Biomedicine, Department of Infectious Medicine(Swepub:gu)xolosi
(author)
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Wandall, H. H.
(author)
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Göteborgs universitetInstitutionen för biomedicin, avdelningen för infektionssjukdomar
(creator_code:org_t)
Related titles
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In:Plos Pathogens: Public Library of Science (PLoS)11:41553-73661553-7374
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