Roles of miRNAs in microcystin-LR-induced Sertoli cell toxicity

Zhou, Y.Nanjing University, Peoples R China; Nanjing University, Peoples R China; Nanjing University, Peoples R China (författare)

Funding Agencies|National Natural Science Foundation of China [31370524, 31200401, 21377052, 81170054]; Natural Science Foundation of Jiangsu Province of China [BK2012307, BK20131281, BK2011570]; Specialized Research Fund for Doctoral Program of Higher Education (SRFDP) of Peoples Republic of China [20130091110050]; Open Research Fund of State Key Laboratory of Bioelectronics, Southeast University [G5]
Microcystin (MC)-LR, a cyclic heptapeptide, is a potent reproductive system toxin. To understand the molecular mechanisms of MC-induced reproductive system cytotoxicity, we evaluated global changes of miRNA and mRNA expression in mouse Sertoli cells following MC-LR treatment. Our results revealed that the exposure to MC-LR resulted in an altered miRNA expression profile that might be responsible for the modulation of mRNA expression. Bio-functional analysis indicated that the altered genes were involved in specific cellular processes, including cell death and proliferation. Target gene analysis suggested that junction injury in Sertoli cells exposed to MC-LR might be mediated by miRNAs through the regulation of the Sertoli cell-Sertoli cell pathway. Collectively, these findings may enhance our understanding on the modes of action of MC-LR on mouse Sertoli cells as well as the molecular mechanisms underlying the toxicity of MC-LR on the male reproductive system. (C) 2015 Elsevier Inc. All rights reserved.

MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Farmakologi och toxikologi hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine Pharmacology and Toxicology hsv//eng
MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin hsv//swe
MEDICAL AND HEALTH SCIENCES Clinical Medicine hsv//eng
Microcystin-LR
microRNA
mRNA
Sertoli cell
Bioinformatics
Toxicity
PROTEIN ZO-1 ESTABLISHES
MICRORNA EXPRESSION
ACTIN CYTOSKELETON
RAT
HEPATOCYTES
IN-VITRO
OCCLUDIN
MICE
CYANOBACTERIA
NONYLPHENOL
APOPTOSIS
Pharmacology & Pharmacy
Toxicology
Microcystin-LR; microRNA; mRNA; Sertoli cell; Bioinformatics; Toxicity

Wang, HuiLinköpings universitet,Avdelningen för kliniska vetenskaper,Medicinska fakulteten(Swepub:liu)huiwa84 (författare)
Wang, C.Nanjing University, Peoples R China; Nanjing University, Peoples R China; Nanjing University, Peoples R China (författare)
Qiu, X. F.Nanjing University, Peoples R China (författare)
Benson, MikaelLinköpings universitet,Avdelningen för kliniska vetenskaper,Medicinska fakulteten,Region Östergötland, Allergicentrum US(Swepub:liu)mikbe05 (författare)
Yin, X. Q.Nanjing University, Peoples R China; Nanjing University, Peoples R China; Nanjing University, Peoples R China (författare)
Xiang, ZouGothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi,Institute of Biomedicine, Department of Microbiology and Immunology,University of Gothenburg, Sweden(Swepub:gu)xxiazo (författare)
Li, D. M.Nanjing University, Peoples R China; Nanjing University, Peoples R China; Nanjing University, Peoples R China (författare)
Han, X. D.Nanjing University, Peoples R China; Nanjing University, Peoples R China; Nanjing University, Peoples R China (författare)
Nanjing University, Peoples R China; Nanjing University, Peoples R China; Nanjing University, Peoples R ChinaAvdelningen för kliniska vetenskaper (creator_code:org_t)

Ingår i:Toxicology and Applied Pharmacology: Elsevier BV287:1, s. 1-80041-008X1096-0333

Av författaren/redakt...: Zhou, Y.; Wang, Hui; Wang, C.; Qiu, X. F.; Benson, Mikael; Yin, X. Q.; visa fler...; Xiang, Zou; Li, D. M.; Han, X. D.; visa färre...

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