Roles of miRNAs in microcystin-LR-induced Sertoli cell toxicity

• Microcystin (MC)-LR, a cyclic heptapeptide, is a potent reproductive system toxin. To understand the molecular mechanisms of MC-induced reproductive system cytotoxicity, we evaluated global changes of miRNA and mRNA expression in mouse Sertoli cells following MC-LR treatment. Our results revealed that the exposure to MC-LR resulted in an altered miRNA expression profile that might be responsible for the modulation of mRNA expression. Bio-functional analysis indicated that the altered genes were involved in specific cellular processes, including cell death and proliferation. Target gene analysis suggested that junction injury in Sertoli cells exposed to MC-LR might be mediated by miRNAs through the regulation of the Sertoli cell-Sertoli cell pathway. Collectively, these findings may enhance our understanding on the modes of action of MC-LR on mouse Sertoli cells as well as the molecular mechanisms underlying the toxicity of MC-LR on the male reproductive system. (C) 2015 Elsevier Inc. All rights reserved.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Farmakologi och toxikologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Pharmacology and Toxicology (hsv//eng)

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine (hsv//eng)

Nyckelord

Microcystin-LR

microRNA

mRNA

Sertoli cell

Bioinformatics

Toxicity

PROTEIN ZO-1 ESTABLISHES

MICRORNA EXPRESSION

ACTIN CYTOSKELETON

RAT

HEPATOCYTES

IN-VITRO

OCCLUDIN

MICE

CYANOBACTERIA

NONYLPHENOL

APOPTOSIS

Pharmacology & Pharmacy

Toxicology

Microcystin-LR; microRNA; mRNA; Sertoli cell; Bioinformatics; Toxicity

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