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Role of Wnt/-Cateni...
Role of Wnt/-Catenin Signaling in Epithelial Differentiation of Lung Resident Mesenchymal Stem Cells
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Shi, C. W. (author)
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Lv, T. F. (author)
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- Xiang, Zou (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi,Institute of Biomedicine, Department of Microbiology and Immunology
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Sun, Z. R. (author)
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Qian, W. P. (author)
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Han, X. D. (author)
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(creator_code:org_t)
- 2015-06-10
- 2015
- English.
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In: Journal of Cellular Biochemistry. - : Wiley. - 0730-2312. ; 116:8, s. 1532-1539
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Abstract
Subject headings
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- Accumulating evidence has demonstrated that stem cells have the ability to repair the lung tissue injuries following either injection of cultured cells or bone marrow transplantation. As a result, increasing attention has focused on the lung resident mesenchymal stem cells (LR-MSCs) for repairing damaged lung tissues. Meanwhile, some studies have revealed that Wnt/-catenin signaling plays an important role in the epithelial differentiation of mesenchymal stem cells (MSCs). In the current study, our aim was to explore the roles of Wnt/-catenin signaling on cell proliferation and epithelial differentiation of LR-MSCs. We have successfully isolated the stem cell antigen (Sca)-1(+)CD45(-)CD31(-) cells which were proposed to be LR-MSCs by magnetic-activated cell sorting (MACS). Furthermore, we demonstrated the expression of epithelial markers on LR-MSCs following indirect co-culture of these cells with alveolar epithelial type II (ATII) cells, confirming the epithelial phenotype of LR-MSCs following co-culture. In order to clarify the regulatory mechanisms of Wnt/-catenin signaling in epithelial differentiation of LR-MSCs, we measured the protein levels of several important members involved in Wnt/-catenin signaling in the presence or absence of some canonical activators and inhibitors of the -catenin pathways. In conclusion, our study demonstrated that Wnt/-catenin signaling may be an essential mechanism underlying the regulation of epithelial differentiation of LR-MSCs. J. Cell. Biochem. 116: 1532-1539, 2015. (c) 2015 Wiley Periodicals, Inc.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- LUNG RESIDENT MESENCHYMAL STEM CELLS (LR-MSCs)
- DIFFERENTIATION
- ALVEOLAR EPITHELIAL
- SUPPRESSES OSTEOGENIC DIFFERENTIATION
- PROMOTES PROLIFERATION
- STROMAL
- CELLS
- PROGENITOR
- DICKKOPF-1
- MORPHOGENESIS
- ADIPOGENESIS
- POPULATION
- INHIBITION
- NICHES
- Biochemistry & Molecular Biology
- Cell Biology
Publication and Content Type
- ref (subject category)
- art (subject category)
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