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Quantitative Measur...
Quantitative Measurement of Transmitters in Individual Vesicles in the Cytoplasm of Single Cells with Nanotip Electrodes
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- Li, Xianchan, 1982 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,Chalmers tekniska högskola,Chalmers University of Technology,University of Gothenburg
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- Majdi, Soodabeh, 1980 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Dunevall, Johan, 1984 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Mashadi Fathali, Hoda, 1983 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Ewing, Andrew G, 1957 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,Chalmers tekniska högskola,Chalmers University of Technology,University of Gothenburg
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(creator_code:org_t)
- 2015-08-12
- 2015
- Engelska.
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Ingår i: Angewandte Chemie International Edition. - : Wiley. - 1433-7851 .- 1521-3773. ; 54:41, s. 11978-11982
- Relaterad länk:
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https://europepmc.or...
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http://dx.doi.org/10...
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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https://research.cha...
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Abstract
Ämnesord
Stäng
- The quantification of vesicular transmitter content is important for studying the mechanisms of neurotransmission and malfunction in disease, and yet it is incredibly difficult to measure the tiny amounts of neurotransmitters in the attoliter volume of a single vesicle, especially in the cell environment. We introduce a novel method, intracellular vesicle electrochemical cytometry. A nanotip conical carbon-fiber microelectrode was used to electrochemically measure the total content of electroactive neurotransmitters in individual nanoscale vesicles in single PC12 cells as these vesicles lysed on the electrode inside the living cell. The results demonstrate that only a fraction of the quantal neurotransmitter content is released during exocytosis. These data support the intriguing hypothesis that the vesicle does not open all the way during the normal exocytosis process, thus resulting in incomplete expulsion of the vesicular contents.
Ämnesord
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
Nyckelord
- catecholamines
- electrochemistry
- exocytosis
- nanoelectrodes
- vesicles
- Carbon
- Carbon fibers
- Microelectrodes
- Nanotips
- Neurophysiology
- Transmitters
- Conical carbon
- Electroactive neurotransmitters
- Intracellular vesicles
- Nano-electrodes
- Quantitative measurement
- Electrodes
- Carbon fibers
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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