Human adipose tissue expresses angiotensinogen and enzymes required for its conversion to angiotensin II.

Angiotensin II regulates blood pressure and may affect adipogenesis and adipocyte metabolism. Angiotensin II is produced by cleavage of angiotensinogen by renin and angiotensin-converting enzyme in the circulation. In addition, angiotensin II may be produced in various tissues by enzymes of the renin-angiotensin system (RAS) or the nonrenin-angiotensin system (NRAS). We have analyzed the expression of angiotensinogen and enzymes required for its conversion to angiotensin II in human adipose tissue. Northern blot demonstrated angiotensinogen expression in adipose tissue from nine obese subjects. Western blot revealed a distinct band of expected size of the angiotensinogen protein (61 kDa) in isolated adipocytes. RT-PCR, followed by Southern blot, demonstrated renin expression in human adipose tissue. Angiotensin-converting enzyme messenger RNA was detected by RT-PCR, and the identity of the PCR products was verified by restriction enzyme cleavage. Transcripts for cathepsin D and cathepsin G, components of the NRAS, were detected by RT-PCR, verified by restriction enzyme cleavage. We conclude that human adipose tissue expresses angiotensinogen and enzymes of RAS and NRAS. This opens the possibility that angiotensinogen-derived peptides, produced in adipose tissue itself, may affect adipogenesis and play a role in the pathogenesis of obesity.

MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Cell- och molekylärbiologi hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine Cell and Molecular Biology hsv//eng
Adipose Tissue
enzymology
metabolism
Adult
Angiotensin II
metabolism
Angiotensinogen
biosynthesis
metabolism
Cathepsin D
metabolism
Cathepsin G
Cathepsins
metabolism
Female
Humans
Middle Aged
Peptidyl-Dipeptidase A
genetics
metabolism
RNA
Messenger
analysis
Renin
genetics
Renin-Angiotensin System
physiology
Reverse Transcriptase Polymerase Chain Reaction
Serine Endopeptidases
metabolism

Lindell, Kajsa,1971Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin,Institute of Internal Medicine (författare)
Ottosson, Malin,1959Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin,Institute of Internal Medicine(Swepub:gu)xlonnm (författare)
Sjöström, LarsGothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin,Institute of Internal Medicine(Swepub:gu)xsjosl (författare)
Carlsson, Björn,1958Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin,Institute of Internal Medicine(Swepub:gu)xcarbj (författare)
Carlsson, Lena M S,1957Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin,Institute of Internal Medicine(Swepub:gu)xcarle (författare)
Göteborgs universitetInstitutionen för invärtesmedicin (creator_code:org_t)

Ingår i:The Journal of clinical endocrinology and metabolism: The Endocrine Society83:11, s. 3925-90021-972X1945-7197

Av författaren/redakt...: Karlsson, C; Lindell, Kajsa, ...; Ottosson, Malin, ...; Sjöström, Lars; Carlsson, Björn, ...; Carlsson, Lena M ...

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MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; och Medicinska och f ...; och Cell och molekyl ...

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