Sökning: WFRF:(Heydenreich M.) >
Antiplasmodial and ...
Antiplasmodial and cytotoxic activities of the constituents of Turraea robusta and Turraea nilotica
-
- Irungu, Beatrice N. (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
-
Adipo, N. (författare)
-
Orwa, J. A. (författare)
-
visa fler...
-
Kimani, F. (författare)
-
Heydenreich, M. (författare)
-
Midiwo, J. O. (författare)
-
- Björemark, Per Martin, 1975 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
-
- Håkansson, Mikael, 1957 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
-
Yenesew, A. (författare)
-
- Erdelyi, Mate, 1975 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,organisk kemi
-
visa färre...
-
(creator_code:org_t)
- Elsevier Ireland Ltd, 2015
- 2015
- Engelska.
-
Ingår i: Journal of Ethnopharmacology. - : Elsevier Ireland Ltd. - 0378-8741 .- 1872-7573. ; 174, s. 419-425
- Relaterad länk:
-
https://doi.org/10.1...
-
visa fler...
-
https://gup.ub.gu.se...
-
https://doi.org/10.1...
-
https://urn.kb.se/re...
-
visa färre...
Abstract
Ämnesord
Stäng
- Ethnopharmacological relevance: Turraea robusta and Turraea nilotica are African medicinal plants used for the treatment of a wide variety of diseases, including malaria. The genus Turraea is rich in limonoids and other triterpenoids known to possess various biological activities. Materials and methods: From the stem bark of T. robusta six compounds, and from various parts of T. nilotica eleven compounds were isolated by the use of a combination of chromatographic techniques. The structures of the isolated compounds were elucidated using NMR and MS, whilst the relative configuration of one of the isolated compounds, toonapubesin F, was established by X-ray crystallography. The antiplasmodial activities of the crude extracts and the isolated constituents against the D6 and W2 strains of Plasmodium falciparum were determined using the semiautomated micro dilution technique that measures the ability of the extracts to inhibit the incorporation of (G-3H, where G is guanine) hypoxanthine into the malaria parasite. The cytotoxicity of the crude extracts and their isolated constituents was evaluated against the mammalian cell lines African monkey kidney (vero), mouse breast cancer (4T1) and human larynx carcinoma (HEp2). Results: The extracts showed good to moderate antiplasmodial activities, where the extract of the stem bark of T. robusta was also cytotoxic against the 4T1 and the HEp2 cells (IC50<10 μg/ml). The compounds isolated from these extracts were characterized as limonoids, protolimonoids and phytosterol glucosides. These compounds showed good to moderate activities with the most active one being azadironolide, IC50 2.4±0.03 μM and 1.1±0.01 μM against the D6 and W2 strains of Plasmodium falciparum, respectively; all other compounds possessed IC50 14.4-40.5 μM. None of the compounds showed significant cytotoxicity against vero cells, yet four of them were toxic against the 4T1 and HEp2 cancer cell lines with piscidinol A having IC50 8.0±0.03 and 8.4 ±0.01 μM against the 4T1 and HEp2 cells, respectively. Diacetylation of piscidinol A resulted in reduced cytotoxicity. Conclusion: From the medicinal plants T. robusta and T. nilotica, twelve compounds were isolated and characterized; two of the isolated compounds, namely 11-epi-toonacilin and azadironolide showed good antiplasmodial activity with the highest selectivity indices. © 2015 The Authors. Published by Elsevier Ireland Ltd.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Farmakologi och toxikologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Pharmacology and Toxicology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Kemi -- Organisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Organic Chemistry (hsv//eng)
Nyckelord
- Antiplasmodial activity
- Azadironolide
- Cytotoxicity
- Limonoid
- Toonacilin
- Toonapubesins F
- Turraea nilotica
- Turraea robusta
- 11 epi toonacillin
- 12alpha acetoxy 7 deacetylazadirone
- 1alpha
- 3alpha diacety 7alpha tigloyvillasinin
- antimalarial agent
- azadirone
- chloroquine
- glucoside
- hispidol B
- hypoxanthine
- mzikonone
- niloticin
- phytosterol
- piscidinol A
- plant extract
- plant medicinal product
- protolimonoid
- sitosterol 3 o beta dextro glucopyranoside
- stigmasterol
- stigmasterol 3 o beta dextro glucopyranoside acetate
- toonapubesin F
- Turraea nilotica extract
- Turraea robusta extract
- turranolide
- unclassified drug
- acetylation
- animal cell
- antimalarial activity
- Article
- bark
- breast cancer
- controlled study
- HEp 2 cell line
- human
- human cell
- larynx carcinoma
- medicinal plant
- mouse
- nonhuman
- Plasmodium falciparum
- Vero cell line
- X ray crystallography
- X ray diffraction
- Antiplasmodial activity
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Irungu, Beatrice ...
-
Adipo, N.
-
Orwa, J. A.
-
Kimani, F.
-
Heydenreich, M.
-
Midiwo, J. O.
-
visa fler...
-
Björemark, Per M ...
-
Håkansson, Mikae ...
-
Yenesew, A.
-
Erdelyi, Mate, 1 ...
-
visa färre...
- Om ämnet
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Medicinska och f ...
-
och Farmakologi och ...
-
- NATURVETENSKAP
-
NATURVETENSKAP
-
och Biologi
-
och Biokemi och mole ...
-
- NATURVETENSKAP
-
NATURVETENSKAP
-
och Kemi
-
och Organisk kemi
- Artiklar i publikationen
-
Journal of Ethno ...
- Av lärosätet
-
Göteborgs universitet
-
Uppsala universitet