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  • Wands, Amberlyn M (author)

Fucosylation and protein glycosylation create functional receptors for cholera toxin.

  • Article/chapterEnglish2015

Publisher, publication year, extent ...

  • 2015

Numbers

  • LIBRIS-ID:oai:gup.ub.gu.se/227524
  • https://gup.ub.gu.se/publication/227524URI
  • https://doi.org/10.7554/eLife.09545DOI

Supplementary language notes

  • Language:English

Part of subdatabase

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  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • Cholera toxin (CT) enters and intoxicates host cells after binding cell surface receptors using its B subunit (CTB). The ganglioside (glycolipid) GM1 is thought to be the sole CT receptor; however, the mechanism by which CTB binding to GM1 mediates internalization of CT remains enigmatic. Here we report that CTB binds cell surface glycoproteins. Relative contributions of gangliosides and glycoproteins to CTB binding depend on cell type, and CTB binds primarily to glycoproteins in colonic epithelial cell lines. Using a metabolically incorporated photocrosslinking sugar, we identified one CTB-binding glycoprotein and demonstrated that the glycan portion of the molecule, not the protein, provides the CTB interaction motif. We further show that fucosylated structures promote CTB entry into a colonic epithelial cell line and subsequent host cell intoxication. CTB-binding fucosylated glycoproteins are present in normal human intestinal epithelia and could play a role in cholera.

Subject headings and genre

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  • Fujita, Akiko (author)
  • McCombs, Janet E (author)
  • Cervin, JakobGothenburg University,Göteborgs universitet,Institutionen för biomedicin,Institute of Biomedicine(Swepub:gu)xnygpa (author)
  • Dedic, BenjaminGothenburg University,Göteborgs universitet,Institutionen för biomedicin,Institute of Biomedicine (author)
  • Rodriguez, Andrea C (author)
  • Nischan, Nicole (author)
  • Bond, Michelle R (author)
  • Mettlen, MarcelGothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi,Institute of Biomedicine, Department of Microbiology and Immunology (author)
  • Trudgian, David C (author)
  • Lemoff, Andrew (author)
  • Quiding-Järbrink, Marianne,1964Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi,Institute of Biomedicine, Department of Microbiology and Immunology(Swepub:gu)xquima (author)
  • Gustavsson, Bengt,1947Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för kirurgi,Institute of Clinical Sciences, Department of Surgery(Swepub:gu)xguben (author)
  • Steentoft, Catharina (author)
  • Clausen, Henrik (author)
  • Mirzaei, Hamid (author)
  • Teneberg, Susann,1955Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology(Swepub:gu)xtensu (author)
  • Yrlid, Ulf,1971Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi,Institute of Biomedicine, Department of Microbiology and Immunology(Swepub:gu)xyrlul (author)
  • Kohler, Jennifer J (author)
  • Göteborgs universitetInstitutionen för biomedicin (creator_code:org_t)

Related titles

  • In:eLife42050-084X

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