Electrochemical Measurements of Optogenetically Stimulated Quantal Amine Release from Single Nerve Cell Varicosities in Drosophila Larvae

Majdi, Soodabeh,1980Chalmers tekniska högskola,Chalmers University of Technology (author)

The nerve terminals found in the body wall of Drosophila melanogaster larvae are readily accessible to experimental manipulation. We used the light-activated ion channel, channelrhodopsin-2, which is expressed by genetic manipulation in TypeII varicosities to study octopamine release in Drosophila. We report the development of a method to measure neurotransmitter release from exocytosis events at individual varicosities in the Drosophila larval system by amperometry. A microelectrode was placed in a region of the muscle containing a varicosity and held at a potential sufficient to oxidize octopamine and the terminal stimulated by blue light. Optical stimulation of TypeII boutons evokes exocytosis of octopamine, which is detected through oxidization at the electrode surface. We observe 22700 +/- 4200 molecules of octopamine released per vesicle. This system provides a genetically accessible platform to study the regulation of amine release at an intact synapse.

Berglund, E CarinaGothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,University of Gothenburg (author)
Dunevall, Johan,1984Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)dunevall (author)
Oleinick, A. I.Processus d'Activation Selective Par Transfert d'Energie Uni-Electronique ou Radiatif (author)
Amatore, C.Processus d'Activation Selective Par Transfert d'Energie Uni-Electronique ou Radiatif (author)
Krantz, D. E.University of California at Los Angeles (UCLA) (author)
Ewing, Andrew G,1957Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,University of Gothenburg,Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)andrewe (author)
Chalmers tekniska högskolaInstitutionen för kemi och molekylärbiologi (creator_code:org_t)

In:Angewandte Chemie International Edition: Wiley54:46, s. 13609-136121433-78511521-3773

By the author/editor: Majdi, Soodabeh, ...; Berglund, E Cari ...; Dunevall, Johan, ...; Oleinick, A. I.; Amatore, C.; Krantz, D. E.; show more...; Ewing, Andrew G, ...; show less...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Cell Biology

By the university: University of Gothenburg; Chalmers University of Technology

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