Loss of the Mediator subunit Med20 affects transcription of tRNA and other non-coding RNA genes in fission yeast

• Mediator is a co-regulator of RNA polymerase II (Pol II), transducing signals from regulatory elements and transcription factors to the general transcription machinery at the promoter. We here demonstrate that Med20 influences ribosomal protein expression in fission yeast. In addition, loss of Med20 leads to an accumulation of aberrant, readthrough tRNA transcripts. These transcripts are polyadenylated and targeted for degradation by the exosome. Similarly, other non-coding RNA molecules, such as snRNA, snoRNA and rRNA, are also enriched in the polyadenylate preparations in the absence of Med20. We suggest that fission yeast Mediator takes part in a regulatory pathway that affects Pol III-dependent transcripts.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinsk bioteknologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Medical Biotechnology (hsv//eng)

Nyckelord

Transcription

Mediator

Exosome

tRNA

RNA polymerase III

polymerase-iii transcription

schizosaccharomyces-pombe

quality-control

head module

saccharomyces-cerevisiae

tramp complex

la protein

exosome

polyadenylation

degradation

Biochemistry & Molecular Biology

Biophysics

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)