Delivery of Small Interfering RNAs to Cells via Exosomes

• Exosomes are small membrane bound vesicles between 30 and 100 nm in diameter of endocytic origin that are secreted into the extracellular environment by many different cell types. Exosomes play a role in intercellular communication by transferring proteins, lipids, and RNAs to recipient cells.Exosomes from human cells could be used as vectors to provide cells with therapeutic RNAs. Here we describe how exogenous small interfering RNAs may successfully be introduced into various kinds of human exosomes using electroporation and subsequently delivered to recipient cells. Methods used to confirm the presence of siRNA inside exosomes and cells are presented, such as flow cytometry, confocal microscopy, and Northern blot.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine (hsv//eng)

Nyckelord

Blood Buffy Coat/cytology

Blotting

Northern

Blotting

Western

Cell Line

Tumor

Cell Separation

Drug Delivery Systems/*methods

Electroporation/*methods

Exosomes/*metabolism

Flow Cytometry

Humans

Lymphocytes/cytology

Microscopy

Confocal

Monocytes/cytology

RNA

Small Interfering/*metabolism

Electroporation

Exosome delivery

Exosome uptake

Exosomes

Extracellular vesicles

Plasma exosomes

Post-transcriptional gene silencing

siRNA delivery

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)