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  • Asplund, Annika,1979-Högskolan i Skövde,Institutionen för biovetenskap,Forskningscentrum för Systembiologi,Takara Bio Europe AB (former Cellartis AB), Gothenburg, Sweden,Bioinformatik, Bioinformatics (author)

One Standardized Differentiation Procedure Robustly Generates Homogenous Hepatocyte Cultures Displaying Metabolic Diversity from a Large Panel of Human Pluripotent Stem Cells

  • Article/chapterEnglish2016

Publisher, publication year, extent ...

  • 2015-09-19
  • Springer Science and Business Media LLC,2016

Numbers

  • LIBRIS-ID:oai:gup.ub.gu.se/237504
  • https://gup.ub.gu.se/publication/237504URI
  • https://doi.org/10.1007/s12015-015-9621-9DOI
  • https://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-11781URI

Supplementary language notes

  • Language:English

Part of subdatabase

Classification

  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • Human hepatocytes display substantial functional inter-individual variation regarding drug metabolizing functions. In order to investigate if this diversity is mirrored in hepatocytes derived from different human pluripotent stem cell (hPSC) lines, we evaluated 25 hPSC lines originating from 24 different donors for hepatic differentiation and functionality. Homogenous hepatocyte cultures could be derived from all hPSC lines using one standardized differentiation procedure. To the best of our knowledge this is the first report of a standardized hepatic differentiation procedure that is generally applicable across a large panel of hPSC lines without any adaptations to individual lines. Importantly, with regard to functional aspects, such as Cytochrome P450 activities, we observed that hepatocytes derived from different hPSC lines displayed inter-individual variation characteristic for primary hepatocytes obtained from different donors, while these activities were highly reproducible between repeated experiments using the same line. Taken together, these data demonstrate the emerging possibility to compile panels of hPSC-derived hepatocytes of particular phenotypes/genotypes relevant for drug metabolism and toxicity studies. Moreover, these findings are of significance for applications within the regenerative medicine field, since our stringent differentiation procedure allows the derivation of homogenous hepatocyte cultures from multiple donors which is a prerequisite for the realization of future personalized stem cell based therapies.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

  • Pradip, A.Takara Bio Europe AB (former Cellartis AB), Gothenburg, Sweden / Novo Nordisk A/S, Bagsværd, Denmark (author)
  • Van Giezen, M.Takara Bio Europe AB (former Cellartis AB), Gothenburg, Sweden (author)
  • Aspegren, A.Takara Bio Europe AB (former Cellartis AB), Gothenburg, Sweden (author)
  • Choukair, H.Takara Bio Europe AB (former Cellartis AB), Gothenburg, Sweden (author)
  • Rehnstrom, M.Takara Bio Europe AB (former Cellartis AB), Gothenburg, Sweden (author)
  • Ghosheh, NidalHögskolan i Skövde,Institutionen för biovetenskap,Forskningscentrum för Systembiologi,Bioinformatik, Bioinformatics(Swepub:his)ghon (author)
  • El Hajjam, D.Takara Bio Europe AB (former Cellartis AB), Gothenburg, Sweden (author)
  • Holmgren, SandraGothenburg University,Göteborgs universitet,Institutionen för biomedicin,Institute of Biomedicine,Takara Bio Europe AB (former Cellartis AB), Gothenburg, Sweden / Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden(Swepub:gu)xhosan (author)
  • Larsson, S.Takara Bio Europe AB (former Cellartis AB), Gothenburg, Sweden (author)
  • Benecke, J.Takara Bio Europe AB (former Cellartis AB), Gothenburg, Sweden (author)
  • Butron, M.Takara Bio Europe AB (former Cellartis AB), Gothenburg, Sweden (author)
  • Wigander, A.Takara Bio Europe AB (former Cellartis AB), Gothenburg, Sweden (author)
  • Noaksson, KarinTakara Bio Europe AB (former Cellartis AB), Gothenburg, Sweden (author)
  • Sartipy, PeterHögskolan i Skövde,Institutionen för biovetenskap,Forskningscentrum för Systembiologi,AstraZeneca R&D, GMD CVMD GMed, Mölndal, Sweden,Bioinformatik, Bioinformatics(Swepub:his)sarp (author)
  • Bjorquist, P.Takara Bio Europe AB (former Cellartis AB), Gothenburg, Sweden / NovaHep AB, Gothenburg, Sweden (author)
  • Edsbagge, J.Takara Bio Europe AB (former Cellartis AB), Gothenburg, Sweden (author)
  • Küppers-Munther, BarbaraHögskolan i Skövde,Institutionen för biovetenskap,Forskningscentrum för Systembiologi,Takara Bio Europe AB (former Cellartis AB), Arvid Wallgrens Backe 20, 413 46, Gothenburg, Sweden(Swepub:his)kupb (author)
  • Jacobsson, SusannaTakara Bio Europe AB (former Cellartis AB), Gothenburg, Sweden (author)
  • Högskolan i SkövdeInstitutionen för biovetenskap (creator_code:org_t)

Related titles

  • In:Stem Cell Reviews and Reports: Springer Science and Business Media LLC12:1, s. 90-1041550-89431558-6804

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