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Exosomes in the nose induce immune cell trafficking and harbour an altered protein cargo in chronic airway inflammation.

Lässer, Cecilia, 1981 (författare)
Gothenburg University,Göteborgs universitet,Krefting Research Centre
O'Neil, Serena, 1981 (författare)
Gothenburg University,Göteborgs universitet,Krefting Research Centre
Shelke, Ganesh V, 1986 (författare)
Gothenburg University,Göteborgs universitet,Krefting Research Centre
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Sihlbom, Carina, 1973 (författare)
Gothenburg University,Göteborgs universitet,Core Facilities, Proteomics,Core Facilities, Proteomics
Folkesson Hansson, Sara, 1976 (författare)
Gothenburg University,Göteborgs universitet,Core Facilities, Proteomics,Core Facilities, Proteomics
Gho, Yong Song, 1964 (författare)
Lundbäck, Bo, 1948 (författare)
Gothenburg University,Göteborgs universitet,Krefting Research Centre
Lötvall, Jan, 1956 (författare)
Gothenburg University,Göteborgs universitet,Krefting Research Centre
visa färre...
 (creator_code:org_t)
2016-06-20
2016
Engelska.
Ingår i: Journal of Translational Medicine. - : Springer Science and Business Media LLC. - 1479-5876. ; 14:1
  • Tidskriftsartikel (refereegranskat)
Abstract Ämnesord
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  • BACKGROUND: Exosomes are nano-sized extracellular vesicles participating in cell-to-cell communication both in health and disease. However, the knowledge about the functions and molecular composition of exosomes in the upper airways is limited. The aim of the current study was therefore to determine whether nasal exosomes can influence inflammatory cells and to establish the proteome of nasal lavage fluid-derived exosomes in healthy subjects, as well as its alterations in individuals with chronic airway inflammatory diseases [asthma and chronic rhinosinusitis (CRS)]. METHODS: Nasal lavage fluid was collected from 14 healthy subjects, 15 subjects with asthma and 13 subjects with asthma/CRS. Exosomes were isolated with differential centrifugation and the proteome was analysed by LC-MS/MS with the application of two exclusion lists as well as using quantitative proteomics. Ingenuity Pathways Analysis and GO Term finder was used to predict the functions associated with the exosomal proteome and a migration assay was used to analyse the effect on immune cells by nasal exosomes. RESULTS: Firstly, we demonstrate that nasal exosomes can induce migration of several immune cells, such as monocytes, neutrophils and NK cells in vitro. Secondly, a mass spectrometry approach, with the application of exclusion lists, was utilised to generate a comprehensive protein inventory of the exosomes from healthy subjects. The use of exclusion lists resulted in the identification of ~15 % additional proteins, and increased the confidence in ~20 % of identified proteins. In total, 604 proteins were identified in nasal exosomes and the nasal exosomal proteome showed strong associations with immune-related functions, such as immune cell trafficking. Thirdly, a quantitative proteomics approach was used to determine alterations in the exosome proteome as a result of airway inflammatory disease. Serum-associated proteins and mucins were more abundant in the exosomes from subjects with respiratory diseases compared to healthy controls while proteins with antimicrobial functions and barrier-related proteins had decreased expression. CONCLUSIONS: Nasal exosomes were shown to induce the migration of innate immune cells, which may be important as the airway epithelium is the first line of defence against pathogens and allergens. The decreased expression in barrier and antimicrobial exosomal proteins in subjects with airway diseases, could possibly contribute to an increased susceptibility to infections, which have important clinical implications in disease progression.

Ämnesord

NATURVETENSKAP  -- Biologi -- Cellbiologi (hsv//swe)
NATURAL SCIENCES  -- Biological Sciences -- Cell Biology (hsv//eng)
MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Lungmedicin och allergi (hsv//swe)
MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Respiratory Medicine and Allergy (hsv//eng)

Nyckelord

Asthma
Chronic rhinosinusitis
Exclusion list
Exosomes
Extracellular vesicles
Cell migration
Mass spectrometry
Nasal lavage fluid
Proteomics
Tandem mass tags

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