onr:"swepub:oai:gup.ub.gu.se/239532"
Search: onr:"swepub:oai:gup.ub.gu.se/239532" > Global Mapping of O...
- 1 of 1
- Previous record
- Next record
- To hitlist
- Bagdonaite, I. (author)
Global Mapping of O-Glycosylation of Varicella Zoster Virus, Human Cytomegalovirus, and Epstein-Barr Virus
- Article/chapterEnglish2016
Publisher, publication year, extent ...
- 2016
Numbers
- LIBRIS-ID:oai:gup.ub.gu.se/239532
- https://gup.ub.gu.se/publication/239532URI
- https://doi.org/10.1074/jbc.M116.721746DOI
Supplementary language notes
- Language:English
Part of subdatabase
- SwePubSwePub
Classification
Notes
- Herpesviruses are among the most complex and widespread viruses, infection and propagation of which depend on envelope proteins. These proteins serve as mediators of cell entry as well as modulators of the immune response and are attractive vaccine targets. Although envelope proteins are known to carry glycans, little is known about the distribution, nature, and functions of these modifications. This is particularly true for O-glycans; thus we have recently developed a "bottom up" mass spectrometry-based technique for mapping O-glycosylation sites on herpes simplex virus type 1. We found wide distribution of O-glycans on herpes simplex virus type 1 glycoproteins and demonstrated that elongated O-glycans were essential for the propagation of the virus. Here, we applied our proteome-wide discovery platform for mapping O-glycosites on representative and clinically significant members of the herpesvirus family: varicella zoster virus, human cytomegalovirus, and Epstein-Barr virus. We identified a large number of O-glycosites distributed on most envelope proteins in all viruses and further demonstrated conserved patterns of O-glycans on distinct homologous proteins. Because glycosylation is highly dependent on the host cell, we tested varicella zoster virus-infected cell lysates and clinically isolated virus and found evidence of consistent O-glycosites. These results present a comprehensive view of herpesvirus O-glycosylation and point to the widespread occurrence of O-glycans in regions of envelope proteins important for virus entry, formation, and recognition by the host immune system. This knowledge enables dissection of specific functional roles of individual glycosites and, moreover, provides a framework for design of glycoprotein vaccines with representative glycosylation.
Subject headings and genre
- MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Infektionsmedicin hsv//swe
- MEDICAL AND HEALTH SCIENCES Clinical Medicine Infectious Medicine hsv//eng
- herpes-simplex-virus
- cell-cell fusion
- insulin-degrading enzyme
- transferase gene family
- n-linked glycans
- glycoprotein b
- envelope
- protein
- epithelial-cells
- viral replication
- crystal-structure
- Biochemistry & Molecular Biology
Added entries (persons, corporate bodies, meetings, titles ...)
- Nordén, Rickard,1977Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Institute of Biomedicine, Department of Infectious Medicine(Swepub:gu)xnorri (author)
- Joshi, H. J. (author)
- King, S. L. (author)
- Vakhrushev, S. Y. (author)
- Olofsson, Sigvard,1948Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Institute of Biomedicine, Department of Infectious Medicine(Swepub:gu)xolosi (author)
- Wandall, H. H. (author)
- Göteborgs universitetInstitutionen för biomedicin, avdelningen för infektionssjukdomar (creator_code:org_t)
Related titles
- In:Journal of Biological Chemistry291:23, s. 12014-120280021-9258
Internet link
Find in a library
- Journal of Biological Chemistry (Search for host publication in LIBRIS)
To the university's database
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Bagdonaite, I.
- Nordén, Rickard, ...
- Joshi, H. J.
- King, S. L.
- Vakhrushev, S. Y ...
- Olofsson, Sigvar ...
- show more...
- Wandall, H. H.
- show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
- MEDICAL AND HEAL ...
- and Clinical Medicin ...
- and Infectious Medic ...
- Articles in the publication
- Journal of Biolo ...
- By the university
- University of Gothenburg
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
