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Structural photoact...
Structural photoactivation of a full-length bacterial phytochrome
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- Björling, Alexander, 1983 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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- Berntsson, Oskar, 1989 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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Lehtivuori, H. (author)
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- Takala, Heikki (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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- Hughes, Ashley J, 1987 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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- Panman, Matthijs R, 1983 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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- Hoernke, Maria (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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- Niebling, Stephan (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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- Henry, Léocadie (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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Henning, R. (author)
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Kosheleva, I. (author)
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Chukharev, V. (author)
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Tkachenko, N. V. (author)
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Menzel, A. (author)
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Newby, G. (author)
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Khakhulin, D. (author)
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Wulff, M. (author)
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Ihalainen, J. A. (author)
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- Westenhoff, Sebastian, 1978 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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(creator_code:org_t)
- American Association for the Advancement of Science (AAAS), 2016
- 2016
- English.
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In: Science Advances. - : American Association for the Advancement of Science (AAAS). - 2375-2548. ; 2:8
- Related links:
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https://advances.sci...
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Phytochromes are light sensor proteins found in plants, bacteria, and fungi. They function by converting a photon absorption event into a conformational signal that propagates from the chromophore through the entire protein. However, the structure of the photoactivated state and the conformational changes that lead to it are not known. We report time-resolved x-ray scattering of the full-length phytochrome from Deinococcus radiodurans on micro-and millisecond time scales. We identify a twist of the histidine kinase output domains with respect to the chromophore-binding domains as the dominant change between the photoactivated and resting states. The time-resolved data further show that the structural changes up to the microsecond time scales are small and localized in the chromophore-binding domains. The global structural change occurs within a few milliseconds, coinciding with the formation of the spectroscopic meta-Rc state. Our findings establish key elements of the signaling mechanism of full-length bacterial phytochromes.
Subject headings
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Keyword
- x-ray-scattering
- chromophore-binding domain
- induced proton release
- small-angle scattering
- free-electron laser
- histidine kinases
- deinococcus-radiodurans
- crystal-structure
- flash-photolysis
- ground-state
- Science & Technology - Other Topics
Publication and Content Type
- ref (subject category)
- art (subject category)
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To the university's database
- By the author/editor
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Björling, Alexan ...
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Berntsson, Oskar ...
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Lehtivuori, H.
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Takala, Heikki
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Hughes, Ashley J ...
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Panman, Matthijs ...
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show more...
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Hoernke, Maria
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Niebling, Stepha ...
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Henry, Léocadie
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Henning, R.
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Kosheleva, I.
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Chukharev, V.
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Tkachenko, N. V.
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Menzel, A.
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Newby, G.
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Khakhulin, D.
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Wulff, M.
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Ihalainen, J. A.
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Westenhoff, Seba ...
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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Science Advances
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University of Gothenburg