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Interleukin-16-prod...
Interleukin-16-producing NK cells and T-cells in the blood of tobacco smokers with and without COPD
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- Andersson, Anders (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för invärtesmedicin och klinisk nutrition,Institute of Medicine, Department of Internal Medicine and Clinical Nutrition
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- Malmhäll, Carina, 1959 (författare)
- Gothenburg University,Göteborgs universitet,Krefting Research Centre
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- Houltz, Birgitta, 1952 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för invärtesmedicin och klinisk nutrition,Institute of Medicine, Department of Internal Medicine and Clinical Nutrition
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- Tengvall, Sara, 1977 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för invärtesmedicin och klinisk nutrition,Institute of Medicine, Department of Internal Medicine and Clinical Nutrition
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- Sjöstrand, Margareta, 1947 (författare)
- Gothenburg University,Göteborgs universitet,Krefting Research Centre
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- Qvarfordt, Ingemar, 1954 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för invärtesmedicin och klinisk nutrition,Institute of Medicine, Department of Internal Medicine and Clinical Nutrition
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Lindén, Anders, 1961 (författare)
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- Bossios, Apostolos, 1969 (författare)
- Gothenburg University,Göteborgs universitet,Krefting Research Centre
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(creator_code:org_t)
- 2016-09
- 2016
- Engelska.
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Ingår i: International Journal of Chronic Obstructive Pulmonary Disease. - : Informa UK Limited. - 1178-2005. ; 11, s. 2245-2258
- Relaterad länk:
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https://www.dovepres...
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https://gup.ub.gu.se...
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https://doi.org/10.2...
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Abstract
Ämnesord
Stäng
- Background: Long-term exposure to tobacco smoke causes local inflammation in the airways that involves not only innate immune cells, including NK cells, but also adaptive immune cells such as cytotoxic (CD8(+)) and helper (CD4(+)) T-cells. We have previously demonstrated that long-term tobacco smoking increases extracellular concentration of the CD4(+)-recruiting cytokine interleukin (IL)-16 locally in the airways. Here, we hypothesized that tobacco smoking alters IL-16 biology at the systemic level and that this effect involves oxygen free radicals (OFR). Methods: We quantified extracellular IL-16 protein (ELISA) and intracellular IL-16 in NK cells, T-cells, B-cells, and monocytes (flow cytometry) in blood samples from long-term tobacco smokers with and without chronic obstructive pulmonary disease (COPD) and in never-smokers. NK cells from healthy blood donors were stimulated with water-soluble tobacco smoke components (cigarette smoke extract) with or without an OFR scavenger (glutathione) in vitro and followed by quantification of IL-16 protein. Results: The extracellular concentrations of IL-16 protein in blood did not display any substantial differences between groups. Notably, intracellular IL-16 protein was detected in all types of blood leukocytes. All long-term smokers displayed a decrease in this IL-16 among NK cells, irrespective of COPD status. Further, both NK and CD4(+) T-cell concentrations displayed a negative correlation with pack-years. Moreover, cigarette smoke extract caused release of IL-16 protein from NK cells in vitro, and this was not affected by glutathione, in contrast to the decrease in intracellular IL-16, which was prevented by this drug. Conclusion: Long-term exposure to tobacco smoke does not markedly alter extracellular concentrations of IL-16 protein in blood. However, it does decrease the intracellular IL-16 concentrations in blood NK cells, the latter effect involving OFR. Thus, long-term tobacco smoking exerts an impact at the systemic level that involves NK cells; innate immune cells that are critical for host defense against viruses and tumors-conditions that are over-represented among smokers.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine (hsv//eng)
Nyckelord
- COPD
- interleukin-16
- NK cells
- CD4(+) cells
- CD8(+) cells
- CD69(+)
- obstructive pulmonary-disease
- natural-killer-cells
- peripheral-blood
- airway inflammation
- cigarette-smoking
- lymphocytes
- lung
- expression
- cytokine
- standardization
- Respiratory System
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Andersson, Ander ...
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Malmhäll, Carina ...
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Houltz, Birgitta ...
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Tengvall, Sara, ...
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Sjöstrand, Marga ...
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Qvarfordt, Ingem ...
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Lindén, Anders, ...
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Bossios, Apostol ...
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