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The room temperatur...
The room temperature crystal structure of a bacterial phytochrome determined by serial femtosecond crystallography
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- Edlund, Petra (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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- Takala, Heikki (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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- Claesson, Elin, 1989 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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- Henry, Léocadie (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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- Dods, Robert, 1989 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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Lehtivuori, Heli (author)
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- Panman, Matthijs R, 1983 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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Pande, K. (author)
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White, T. (author)
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Nakane, T. (author)
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- Berntsson, Oskar, 1989 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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- Gustavsson, Emil, 1987 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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- Båth, Petra, 1988 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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Modi, V. (author)
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Roy-Chowdhury, S. (author)
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Zook, J. (author)
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Berntsen, P. (author)
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Pandey, S. (author)
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Poudyal, I. (author)
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Tenboer, J. (author)
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Kupitz, C. (author)
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Barty, A. (author)
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Fromme, P. (author)
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Koralek, J. D. (author)
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Tanaka, T. (author)
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Spence, J. (author)
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Liang, M. L. (author)
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Hunter, M. S. (author)
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Boutet, S. (author)
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Nango, E. (author)
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Moffat, K. (author)
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Groenhof, G. (author)
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Ihalainen, J. (author)
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Stojkovic, E. A. (author)
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Schmidt, M. (author)
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- Westenhoff, Sebastian, 1978 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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(creator_code:org_t)
- 2016-10-19
- 2016
- English.
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In: Scientific Reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 6
- Related links:
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https://www.nature.c...
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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Abstract
Subject headings
Close
- Phytochromes are a family of photoreceptors that control light responses of plants, fungi and bacteria. A sequence of structural changes, which is not yet fully understood, leads to activation of an output domain. Time-resolved serial femtosecond crystallography (SFX) can potentially shine light on these conformational changes. Here we report the room temperature crystal structure of the chromophore-binding domains of the Deinococcus radiodurans phytochrome at 2.1 angstrom resolution. The structure was obtained by serial femtosecond X-ray crystallography from microcrystals at an X-ray free electron laser. We find overall good agreement compared to a crystal structure at 1.35 angstrom resolution derived from conventional crystallography at cryogenic temperatures, which we also report here. The thioether linkage between chromophore and protein is subject to positional ambiguity at the synchrotron, but is fully resolved with SFX. The study paves the way for time-resolved structural investigations of the phytochrome photocycle with time-resolved SFX.
Subject headings
- NATURVETENSKAP -- Biologi -- Strukturbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Structural Biology (hsv//eng)
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Keyword
- CHROMOPHORE-BINDING DOMAIN
- X-RAY-DIFFRACTION
- GROUND-STATE
- RED LIGHT
- PROTEIN
- BACTERIOPHYTOCHROME
- PHOTOCONVERSION
- REARRANGEMENTS
- TRANSDUCTION
- FLUORESCENCE
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
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Edlund, Petra
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Takala, Heikki
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Claesson, Elin, ...
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Henry, Léocadie
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Dods, Robert, 19 ...
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Lehtivuori, Heli
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show more...
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Panman, Matthijs ...
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Pande, K.
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White, T.
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Nakane, T.
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Berntsson, Oskar ...
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Gustavsson, Emil ...
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Båth, Petra, 198 ...
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Modi, V.
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Roy-Chowdhury, S ...
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Zook, J.
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Berntsen, P.
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Pandey, S.
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Poudyal, I.
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Tenboer, J.
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Kupitz, C.
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Barty, A.
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Fromme, P.
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Koralek, J. D.
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Tanaka, T.
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Spence, J.
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Liang, M. L.
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Hunter, M. S.
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Boutet, S.
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Nango, E.
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Moffat, K.
-
Groenhof, G.
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Ihalainen, J.
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Stojkovic, E. A.
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Schmidt, M.
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Westenhoff, Seba ...
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Structural Biolo ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Chemical Science ...
- Articles in the publication
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Scientific Repor ...
- By the university
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University of Gothenburg