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Sökning: (WFRF:(Shelke Ganesh V 1986)) > Two distinct extrac...

  • Lässer, Cecilia,1981Gothenburg University,Göteborgs universitet,Krefting Research Centre (författare)

Two distinct extracellular RNA signatures released by a single cell type identified by microarray and next-generation sequencing.

  • Artikel/kapitelEngelska2017

Förlag, utgivningsår, omfång ...

  • 2016-11-28
  • Informa UK Limited,2017

Nummerbeteckningar

  • LIBRIS-ID:oai:gup.ub.gu.se/245684
  • https://gup.ub.gu.se/publication/245684URI
  • https://doi.org/10.1080/15476286.2016.1249092DOI

Kompletterande språkuppgifter

  • Språk:engelska

Ingår i deldatabas

Klassifikation

  • Ämneskategori:ref swepub-contenttype
  • Ämneskategori:art swepub-publicationtype

Anmärkningar

  • Cells secrete extracellular RNA (exRNA) to their surrounding environment and exRNA has been found in many body fluids such as blood, breast milk and cerebrospinal fluid. However, there are conflicting results regarding the nature of exRNA. Here, we have separated 2 distinct exRNA profiles released by mast cells, here termed high-density (HD) and low-density (LD) exRNA. The exRNA in both fractions was characterized by microarray and next-generation sequencing. Both exRNA fractions contained mRNA and miRNA, and the mRNAs in the LD exRNA correlated closely with the cellular mRNA, whereas the HD mRNA did not. Furthermore, the HD exRNA was enriched in lincRNA, antisense RNA, vault RNA, snoRNA, and snRNA with little or no evidence of full-length 18S and 28S rRNA. The LD exRNA was enriched in mitochondrial rRNA, mitochondrial tRNA, tRNA, piRNA, Y RNA, and full-length 18S and 28S rRNA. The proteomes of the HD and LD exRNA-containing fractions were determined with LC-MS/MS and analyzed with Gene Ontology term finder, which showed that both proteomes were associated with the term extracellular vesicles and electron microscopy suggests that at least a part of the exRNA is associated with exosome-like extracellular vesicles. Additionally, the proteins in the HD fractions tended to be associated with the nucleus and ribosomes, whereas the LD fraction proteome tended to be associated with the mitochondrion. We show that the 2 exRNA signatures released by a single cell type can be separated by floatation on a density gradient. These results show that cells can release multiple types of exRNA with substantial differences in RNA species content. This is important for any future studies determining the nature and function of exRNA released from different cells under different conditions.

Ämnesord och genrebeteckningar

Biuppslag (personer, institutioner, konferenser, titlar ...)

  • Shelke, Ganesh V,1986Gothenburg University,Göteborgs universitet,Krefting Research Centre(Swepub:gu)xshega (författare)
  • Yeri, Ashish (författare)
  • Kim, Dae-Kyum (författare)
  • Crescitelli, Rossella,1985Gothenburg University,Göteborgs universitet,Krefting Research Centre(Swepub:gu)xcrero (författare)
  • Raimondo, StefaniaGothenburg University,Göteborgs universitet,Krefting Research Centre (författare)
  • Sjöstrand, Margareta,1947Gothenburg University,Göteborgs universitet,Krefting Research Centre(Swepub:gu)xsjosm (författare)
  • Gho, Yong Song,1964 (författare)
  • Van Keuren Jensen, Kendall (författare)
  • Lötvall, Jan,1956Gothenburg University,Göteborgs universitet,Krefting Research Centre(Swepub:gu)xlotja (författare)
  • Göteborgs universitetKrefting Research Centre (creator_code:org_t)

Sammanhörande titlar

  • Ingår i:RNA biology: Informa UK Limited14:1, s. 58-721555-85841547-6286

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