Nuclear Magnetic Resonance Structure and Mutational Analysis of the Lactococcin A Immunity Protein.

The class IId bacteriocin lactococcin A and the pediocin-like bacteriocins induce membrane leakage and cell death by specifically binding the mannose phophotransferase system (man-PTS) on their target cells. The bacteriocins' cognate immunity proteins that protect the producer cell from its own bacteriocin recognize and bind to the bacteriocin-man-PTS complex and thereby block membrane leakage. In this study, we have determined the three-dimensional structure of the lactococcin A immunity protein (LciA) by the use of nuclear magnetic resonance spectroscopy. LciA forms a four-helix bundle structure with a flexible C-terminal tail. Despite the low degree of sequence similarity between LciA and the pediocin-like immunity proteins, they share the same fold. However, there are certain differences between the structures. The C-terminal helix in LciA is considerably shorter than that observed in the pediocin-like immunity proteins, and the surface potentials of the immunity proteins differ. Truncated variants of LciA in which 6 or 10 of the C-terminal residues were removed yielded a reduced degree of protection, indicating that the unstructured C-terminal tail is important for the functionality of the immunity proteins.

Persson, Cecilia,1974Gothenburg University,Göteborgs universitet,Svenskt NMR-centrum vid Göteborgs universitet,Swedish NMR Centre at Göteborg University(Swepub:gu)xengmc (författare)
Fuochi, Virginia (författare)
Pedersen, Anders,1976Gothenburg University,Göteborgs universitet,Svenskt NMR-centrum vid Göteborgs universitet,Swedish NMR Centre at Göteborg University(Swepub:gu)xpedan (författare)
Karlsson, B Göran,1962Gothenburg University,Göteborgs universitet,Svenskt NMR-centrum vid Göteborgs universitet,Swedish NMR Centre at Göteborg University(Swepub:gu)xkargo (författare)
Nissen-Meyer, Jon (författare)
Oppegård, Camilla (författare)
Göteborgs universitetSvenskt NMR-centrum vid Göteborgs universitet (creator_code:org_t)

Ingår i:Biochemistry: American Chemical Society (ACS)55:45, s. 6250-62571520-49950006-2960

Av författaren/redakt...: Kristiansen, Per ...; Persson, Cecilia ...; Fuochi, Virginia; Pedersen, Anders ...; Karlsson, B Göra ...; Nissen-Meyer, Jo ...; visa fler...; Oppegård, Camill ...; visa färre...

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