3D reconstruction of biological objects from sequential image planes--applied on cerebral cortex from cat.

• A prism of cat cerebral cortex was reconstructed with a method for three-dimensional (3D) representation of biological objects. A series of 918 semithin sections were digitized into an image analysis system. The images were aligned and analyzed, and a data base with the coordinates and a classification of the cells was created. The data base (i.e., the cortical prism) was visualized in a 3D graphic terminal, and parameters such as columnar and lamellar organization, clustering, and cell density were analyzed. A neuronal perikaryon and its neurites was reconstructed and shown together with the cortical prism.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Neurosciences (hsv//eng)

Nyckelord

Algorithms

Animals

Axons

ultrastructure

Cats

Cell Nucleus

ultrastructure

Cerebral Cortex

anatomy & histology

cytology

Computer Systems

Data Display

Database Management Systems

Dendrites

ultrastructure

Image Processing

Computer-Assisted

methods

Microtomy

Neuroglia

ultrastructure

Neurons

ultrastructure

Software

User-Computer Interface

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)