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Y box-binding prote...
Y box-binding protein-1 binds preferentially to single-stranded nucleic acids and exhibits 3'-->5' exonuclease activity.
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Imamura, T (author)
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Nagatani, G (author)
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Ise, T (author)
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Murakami, T (author)
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Uramoto, H (author)
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Torigoe, T (author)
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Ishiguchi, H (author)
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Yoshida, Y (author)
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Nomoto, M (author)
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Okamoto, T (author)
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Uchiumi, T (author)
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Kuwano, M (author)
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- Funa, Keiko, 1949 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för anatomi och cellbiologi,Institute of Anatomy and Cell Biology
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Kohno, K (author)
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(creator_code:org_t)
- 2001
- 2001
- English.
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In: Nucleic acids research. - 1362-4962. ; 29:5, s. 1200-7
- Related links:
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https://gup.ub.gu.se...
Abstract
Subject headings
Close
- We have previously shown that Y box-binding protein-1 (YB-1) binds preferentially to cisplatin-modified Y box sequences. Based on structural and biochemical data, we predicted that this protein binds single-stranded nucleic acids. In the present study we confirmed the prediction and also discovered some unexpected functional features of YB-1. We found that the cold shock domain of the protein is necessary but not sufficient for double-stranded DNA binding while the C-tail domain interacts with both single-stranded DNA and RNA independently of the cold shock domain. In an in vitro translation system the C-tail domain of the protein inhibited translation but the cold shock domain did not. Both in vitro pull-down and in vivo co-immunoprecipitation assays revealed that YB-1 can form a homodimer. Deletion analysis mapped the C-tail domain of the protein as the region of homodimerization. We also characterized an intrinsic 3'-->5' DNA exonuclease activity of the protein. The region between residues 51 and 205 of its 324-amino acid extent is required for full exonuclease activity. Our findings suggest that YB-1 functions in regulating DNA/RNA transactions and that these actions involve different domains.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- Binding Sites
- Binding
- Competitive
- CCAAT-Enhancer-Binding Proteins
- chemistry
- genetics
- metabolism
- Cisplatin
- pharmacology
- DNA
- metabolism
- DNA
- Single-Stranded
- metabolism
- DNA-Binding Proteins
- Dimerization
- Exonucleases
- metabolism
- Glutathione Transferase
- genetics
- metabolism
- Humans
- Mutation
- NFI Transcription Factors
- Nuclear Proteins
- Oligonucleotides
- metabolism
- Protein Binding
- drug effects
- Protein Biosynthesis
- RNA-Binding Proteins
- genetics
- metabolism
- Recombinant Fusion Proteins
- genetics
- metabolism
- Transcription Factors
- Tumor Cells
- Cultured
- Y-Box-Binding Protein 1
Publication and Content Type
- ref (subject category)
- art (subject category)
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To the university's database
- By the author/editor
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Imamura, T
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Nagatani, G
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Ise, T
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Murakami, T
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Uramoto, H
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Torigoe, T
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show more...
-
Ishiguchi, H
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Yoshida, Y
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Nomoto, M
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Okamoto, T
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Uchiumi, T
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Kuwano, M
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Funa, Keiko, 194 ...
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Kohno, K
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
- Articles in the publication
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Nucleic acids re ...
- By the university
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University of Gothenburg