Expression of platelet-derived growth factor after intrastriatal ibotenic acid injury.

• The expression of platelet-derived growth factor (PDGF) was studied in a rat model of Huntington's disease, produced by unilateral intrastriatal ibotenic acid injections. The most pronounced effect registered was that the number of PDGF-immunoreactive cells increased in the lesioned area up to 10 weeks after the surgery. Double immunofluorescence staining indicated that the PDGF-positive cells were astrocytes. The increased PDGF immunoreactivity was associated with only minor changes in total PDGF mRNA and PDGF protein levels in the lesioned area. Reverse transcription-polymerase chain reaction (RT-PCR) demonstrated a slight increase in PDGF mRNA after ibotenic acid lesion, but this was not reflected in an increase in PDGF A- and B-chain protein concentration as measured with ELISA. After sham operation an increase in PDGF protein concentration was seen, while the number of PDGF-immunoreactive cells was unchanged. The accumulation of PDGF in the astrocytes might reflect the role of PDGF in a repair process in neurodegenerative processes.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine (hsv//eng)

Nyckelord

Animals

Corpus Striatum

drug effects

metabolism

physiology

Disease Models

Animal

Enzyme-Linked Immunosorbent Assay

Female

Huntington Disease

physiopathology

Ibotenic Acid

pharmacology

Immunohistochemistry

Kinetics

Platelet-Derived Growth Factor

genetics

metabolism

Polymerase Chain Reaction

RNA

Messenger

metabolism

Rats

Rats

Sprague-Dawley

Transcription

Genetic

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)