Single cell amperometry reveals curcuminoids modulate the release of neurotransmitters during exocytosis from PC12 cells

Li, Xianchan,1982Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,University of Gothenburg (författare)

We used single cell amperometry to examine whether curcumin and bisdemethoxycurcumin (BDMC), substances that are suggested to affect learning and memory, can modulate monoamine release from PC12 cells. Our results indicate both curcumin and BDMC need long-term treatment (72 h in this study) to influence exocytosis effectively. By analyzing the parameters calculated from single exocytosis events, it can be concluded that curcumin and BDMC affect exocytosis through different mechanisms. Curcumin accelerates the event dynamics with no significant change of the monoamine amount released from single exocytotic events, whereas BDMC attenuates the amount from single exocytotic event with no significant change of the event dynamics. This comparison of the effect of curcumin and BDMC on exocytosis at the single cell level brings insight into their different mechanisms, which might lead to different biological actions. The effect of curcumin and BDMC on the opening and closing of the exocytotic fusion pore were also investigated. These results might be helpful for understanding the improvement of learning and memory and the anti-depression properties of curcuminoids.

NATURVETENSKAP Biologi Biokemi och molekylärbiologi hsv//swe
NATURAL SCIENCES Biological Sciences Biochemistry and Molecular Biology hsv//eng
NATURVETENSKAP Kemi Oorganisk kemi hsv//swe
NATURAL SCIENCES Chemical Sciences Inorganic Chemistry hsv//eng
Single cell amperometry
Exocytosis
Curcumin
Bisdemethoxycurcumin
PC12 cells
Model cells
inhibits glutamate release
fusion pore
electrochemical cytometry
chemical-analysis
chromaffin cells
vesicle release
curcuma-longa
transmitters
events
memory
Chemistry
Electrochemistry
Curcumin

Mohammadi, Amir Saeid,1984Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)saamir (författare)
Ewing, Andrew G,1957Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)andrewe (författare)
Göteborgs universitetInstitutionen för kemi och molekylärbiologi (creator_code:org_t)

Ingår i:Journal of Electroanalytical Chemistry: Elsevier BV781, s. 30-351572-6657

Av författaren/redakt...: Li, Xianchan, 19 ...; Mohammadi, Amir ...; Ewing, Andrew G, ...

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