On the mechanism of electrochemical vesicle cytometry: chromaffin cell vesicles and liposomes

Lovric, Jelena,1980Chalmers tekniska högskola,Chalmers University of Technology (författare)

The mechanism of mammalian vesicle rupture onto the surface of a polarized carbon fiber microelectrode during electrochemical vesicle cytometry is investigated. It appears that following adsorption to the surface of the polarized electrode, electroporation leads to the formation of a pore at the interface between a vesicle and the electrode and this is shown to be potential dependent. The chemical cargo is then released through this pore to be oxidized at the electrode surface. This makes it possible to quantify the contents as it restricts diffusion away from the electrode and coulometric oxidation takes place. Using a bottom up approach, lipid-only transmitter-loaded liposomes were used to mimic native vesicles and the rupture events occurred much faster in comparison with native vesicles. Liposomes with added peptide in the membrane result in rupture events with a lower duration than that of liposomes and faster in comparison to native vesicles. Diffusional models have been developed and suggest that the trend in pore size is dependent on soft nanoparticle size and diffusion of the content in the nanometer vesicle. In addition, it appears that proteins form a barrier for the membrane to reach the electrode and need to move out of the way to allow close contact and electroporation. The protein dense core in vesicles matrixes is also important in the dynamics of the events in that it significantly slows diffusion through the vesicle.

NATURVETENSKAP Biologi Biokemi och molekylärbiologi hsv//swe
NATURAL SCIENCES Biological Sciences Biochemistry and Molecular Biology hsv//eng
NATURVETENSKAP Kemi Analytisk kemi hsv//swe
NATURAL SCIENCES Chemical Sciences Analytical Chemistry hsv//eng
lipid-bilayer formation
fusion pore
amperometric measurements
catecholamine content
vesicular exocytosis
secretory vesicles
extended kiss
full fusion
release
membrane
Chemistry

Najafinobar, Neda,1985Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)nobar (författare)
Dunevall, Johan,1984Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)dunevall (författare)
Majdi, Soodabeh,1980Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)soodabeh (författare)
Svir, I.Processus d'Activation Selective Par Transfert d'Energie Uni-Electronique ou Radiatif (författare)
Oleinick, A.Processus d'Activation Selective Par Transfert d'Energie Uni-Electronique ou Radiatif (författare)
Amatore, C.Processus d'Activation Selective Par Transfert d'Energie Uni-Electronique ou Radiatif (författare)
Ewing, Andrew G,1957Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)andrewe (författare)
Chalmers tekniska högskolaInstitutionen för kemi och molekylärbiologi (creator_code:org_t)

Ingår i:Faraday Discussions: Royal Society of Chemistry (RSC)193, s. 65-791359-66401364-5498

Av författaren/redakt...: Lovric, Jelena, ...; Najafinobar, Ned ...; Dunevall, Johan, ...; Majdi, Soodabeh, ...; Svir, I.; Oleinick, A.; visa fler...; Amatore, C.; Ewing, Andrew G, ...; visa färre...

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