Activation of human neutrophils by mycobacterial phenolic glycolipids.

• The interaction between mycobacterial phenolic glycolipids (PGLs) and phagocytes was studied. Human neutrophils were allowed to interact with each of four purified mycobacterial PGLs and the neutrophil production of reactive oxygen metabolites was followed kinetically by luminol-/isoluminol-amplified chemiluminescence. The PGLs from Mycobacterium tuberculosis and Mycobacterium kansasii, respectively, were shown to stimulate the production of oxygen metabolites, while PGLs from Mycobacterium marinum and Mycobacterium bovis BCG, respectively, were unable to induce an oxidative response. Periodate treatment of the M. tuberculosis PGL decreased the production of oxygen radicals, showing the importance of the PGL carbohydrate moiety for the interaction. The activation, however, could not be inhibited by rhamnose or fucose, indicating a complex interaction which probably involves more than one saccharide unit. This is in line with the fact that the activating PGLs from M. tuberculosis and M. kansasii contain tri- and tetrasaccharides, respectively, while the nonactivating PGLs from M. marinum and M. bovis BCG each contain a monosaccharide. The complement receptor 3 (CR3) has earlier been shown to be of importance for the phagocyte binding of mycobacteria, but did not appear to be involved in the activation of neutrophils by PGLs. The subcellular localization of the reactive oxygen metabolites formed was related to the way in which the glycolipids were presented to the cells.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Microbiology in the medical area (hsv//eng)

Nyckelord

Antigens

Bacterial

immunology

Carbohydrate Metabolism

Carbohydrates

physiology

Complement Inactivator Proteins

pharmacology

Cytoplasmic Granules

metabolism

Cytoskeleton

physiology

Enzyme Induction

immunology

Glycolipids

immunology

Humans

Macrophage-1 Antigen

metabolism

Monosaccharides

pharmacology

Mycobacterium bovis

chemistry

immunology

Mycobacterium kansasii

chemistry

immunology

Mycobacterium marinum

chemistry

immunology

Mycobacterium tuberculosis

chemistry

immunology

NADPH Oxidase

biosynthesis

Neutrophil Activation

Neutrophils

enzymology

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)