Escherichia coli-induced activation of neutrophil NADPH-oxidase: lipopolysaccharide and formylated peptides act synergistically to induce release of reactive oxygen metabolites.

• The prevailing view of neutrophil NADPH-oxidase activation during interaction with bacteria is that the production of toxic oxygen metabolites should be directed into the phagosome containing the engulfed prey. However, in this report we show that a common Escherichia coli strain, HB101, may induce a release of neutrophil oxygen metabolites to the extracellular milieu. This phenomenon is dependent on three factors: (i) the mobilization (upregulation) of neutrophil secretory vesicles prior to interaction with the bacteria, (ii) soluble bacterial factors binding to the formylmethionyl-leucyl-phenylalanine receptor and tentatively identified as formylated peptides, and (iii) a bacterium-associated priming factor identified as lipopolysaccharide.

Subject headings

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Microbiology in the medical area (hsv//eng)

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Immunology in the medical area (hsv//eng)

Keyword

Adult

Cytoplasmic Granules

metabolism

Drug Synergism

Enzyme Activation

Escherichia coli

immunology

Humans

Lipopolysaccharides

pharmacology

Macrophage-1 Antigen

physiology

N-Formylmethionine Leucyl-Phenylalanine

pharmacology

NADH

NADPH Oxidoreductases

metabolism

NADPH Oxidase

Neutrophils

enzymology

Polymyxin B

pharmacology

Reactive Oxygen Species

metabolism

Receptors

Formyl Peptide

Receptors

Immunologic

physiology

Receptors

Peptide

physiology

Publication and Content Type

ref (subject category)

art (subject category)