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From Macrocrystals ...
From Macrocrystals to Microcrystals: A Strategy for Membrane Protein Serial Crystallography.
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- Dods, Robert, 1989 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,Univ Gothenburg, Dept Chem & Mol Biol, Gothenburg, Sweden.
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- Båth, Petra, 1988 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,Univ Gothenburg, Dept Chem & Mol Biol, Gothenburg, Sweden.
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- Arnlund, David (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,Univ Gothenburg, Dept Chem & Mol Biol, Gothenburg, Sweden.
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- Beyerlein, Kenneth R (author)
- Deutsch Elektronen Synchrotron DESY, Ctr Free Electron Laser Sci, Hamburg, Germany.
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- Nelson, Garrett (author)
- Arizona State Univ, Dept Phys, Tempe, AZ 85287 USA.
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- Liang, Mengling (author)
- Stanford Linear Accelerator Ctr SLAC Natl Acceler, Linac Coherent Light Source, Menlo Pk, CA USA.
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- Harimoorthy, Rajiv (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,Univ Gothenburg, Dept Chem & Mol Biol, Gothenburg, Sweden.
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- Berntsen, Peter, 1974 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,Univ Gothenburg, Dept Chem & Mol Biol, Gothenburg, Sweden.;La Trobe Univ, Australian Res Council Ctr Excellence Adv Mol Ima, La Trobe Inst Mol Sci, Bundoora, Vic 3086, Australia.
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- Malmerberg, Erik (author)
- Lawrence Berkeley Natl Lab, Phys Biosci Div, 1 Cyclotron Rd, Berkeley, CA 94720 USA.
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- Johansson, Linda C, 1983 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,Univ Gothenburg, Dept Chem & Mol Biol, Gothenburg, Sweden.;Univ Southern Calif, Bridge Inst, Dept Chem, Los Angeles, CA 90089 USA.
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- Andersson, Rebecka, 1988 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,Univ Gothenburg, Dept Chem & Mol Biol, Gothenburg, Sweden.
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- Bosman, Robert, 1991 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,Univ Gothenburg, Dept Chem & Mol Biol, Gothenburg, Sweden.
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- Carbajo, Sergio (author)
- Stanford Linear Accelerator Ctr SLAC Natl Acceler, Linac Coherent Light Source, Menlo Pk, CA USA.
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- Claesson, Elin, 1989 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,Univ Gothenburg, Dept Chem & Mol Biol, Gothenburg, Sweden.
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- Conrad, Chelsie E (author)
- Arizona State Univ, Dept Phys, Tempe, AZ 85287 USA.
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- Dahl, Peter, 1965 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,Univ Gothenburg, Dept Chem & Mol Biol, Gothenburg, Sweden.
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- Hammarin, Greger, 1981 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,Univ Gothenburg, Dept Chem & Mol Biol, Gothenburg, Sweden.
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- Hunter, Mark S (author)
- Stanford Linear Accelerator Ctr SLAC Natl Acceler, Linac Coherent Light Source, Menlo Pk, CA USA.
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- Li, Chufeng (author)
- Arizona State Univ, Dept Phys, Tempe, AZ 85287 USA.
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- Lisova, Stella (author)
- Arizona State Univ, Dept Phys, Tempe, AZ 85287 USA.
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- Milathianaki, Despina (author)
- Stanford Linear Accelerator Ctr SLAC Natl Acceler, Linac Coherent Light Source, Menlo Pk, CA USA.
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- Robinson, Joseph (author)
- Stanford Linear Accelerator Ctr SLAC Natl Acceler, Linac Coherent Light Source, Menlo Pk, CA USA.
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- Safari, Cecilia, 1989 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,Univ Gothenburg, Dept Chem & Mol Biol, Gothenburg, Sweden.
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- Sharma, Amit (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,Univ Gothenburg, Dept Chem & Mol Biol, Gothenburg, Sweden.
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- Williams, Garth (author)
- Stanford Linear Accelerator Ctr SLAC Natl Acceler, Linac Coherent Light Source, Menlo Pk, CA USA.
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- Wickstrand, Cecilia (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,Univ Gothenburg, Dept Chem & Mol Biol, Gothenburg, Sweden.
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- Yefanov, Oleksandr (author)
- Deutsch Elektronen Synchrotron DESY, Ctr Free Electron Laser Sci, Hamburg, Germany.
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- Davidsson, Jan (author)
- Uppsala universitet,Fysikalisk kemi
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- DePonte, Daniel P (author)
- Deutsch Elektronen Synchrotron DESY, Ctr Free Electron Laser Sci, Hamburg, Germany.
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- Barty, Anton (author)
- Deutsch Elektronen Synchrotron DESY, Ctr Free Electron Laser Sci, Hamburg, Germany.
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- Brändén, Gisela, 1975 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,Univ Gothenburg, Dept Chem & Mol Biol, Gothenburg, Sweden.
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- Neutze, Richard, 1969 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,Univ Gothenburg, Dept Chem & Mol Biol, Gothenburg, Sweden.
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(creator_code:org_t)
- Elsevier BV, 2017
- 2017
- English.
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In: Structure. - : Elsevier BV. - 1878-4186 .- 0969-2126. ; 25:9, s. 1461-1468
- Related links:
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http://www.cell.com/...
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Serial protein crystallography was developed at X-ray free-electron lasers (XFELs) and is now also being applied at storage ring facilities. Robust strategies for the growth and optimization of microcrystals are needed to advance the field. Here we illustrate a generic strategy for recovering high-density homogeneous samples of microcrystals starting from conditions known to yield large (macro) crystals of the photosynthetic reaction center of Blastochloris viridis (RCvir). We first crushed these crystals prior to multiple rounds of microseeding. Each cycle of microseeding facilitated improvements in the RCvir serial femtosecond crystallography (SFX) structure from 3.3-Å to 2.4-Å resolution. This approach may allow known crystallization conditions for other proteins to be adapted to exploit novel scientific opportunities created by serial crystallography.
Subject headings
- NATURVETENSKAP -- Biologi -- Strukturbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Structural Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biofysik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biophysics (hsv//eng)
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
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- art (subject category)
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Structure
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- By the author/editor
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Dods, Robert, 19 ...
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Båth, Petra, 198 ...
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Arnlund, David
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Beyerlein, Kenne ...
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Nelson, Garrett
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Liang, Mengling
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show more...
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Harimoorthy, Raj ...
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Berntsen, Peter, ...
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Malmerberg, Erik
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Johansson, Linda ...
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Andersson, Rebec ...
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Bosman, Robert, ...
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Carbajo, Sergio
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Claesson, Elin, ...
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Conrad, Chelsie ...
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Dahl, Peter, 196 ...
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Hammarin, Greger ...
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Hunter, Mark S
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Li, Chufeng
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Lisova, Stella
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Milathianaki, De ...
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Robinson, Joseph
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Safari, Cecilia, ...
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Sharma, Amit
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Williams, Garth
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Wickstrand, Ceci ...
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Yefanov, Oleksan ...
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Davidsson, Jan
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DePonte, Daniel ...
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Barty, Anton
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Brändén, Gisela, ...
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Neutze, Richard, ...
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Structural Biolo ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biophysics
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Chemical Science ...
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Structure
- By the university
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University of Gothenburg
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Uppsala University