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Interaction of scav...
Interaction of scavenger receptor class B type I with peroxisomal targeting receptor Pex5p.
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- Johnson, Magnus S.C. 1969 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin, Avdelningen för internmedicin,Institute of Internal Medicine, Dept of Medicine
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- Johansson, Jenny M, 1975 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin, Avdelningen för kroppssammansättning och metabolism,Institute of Internal Medicine, Dept of Body Composition and Metabolism
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- Svensson, Per-Arne, 1969 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin, Avdelningen för kroppssammansättning och metabolism,Institute of Internal Medicine, Dept of Body Composition and Metabolism
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- Åberg, Maria A I, 1972 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för klinisk neurovetenskap,Institute of Clinical Neurosciences
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- Eriksson, Peter S, 1959 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för klinisk neurovetenskap,Institute of Clinical Neurosciences
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- Carlsson, Lena M S, 1957 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin, Avdelningen för kroppssammansättning och metabolism,Institute of Internal Medicine, Dept of Body Composition and Metabolism
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- Carlsson, Björn, 1958 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin, Avdelningen för kroppssammansättning och metabolism,Institute of Internal Medicine, Dept of Body Composition and Metabolism
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(creator_code:org_t)
- 2003
- 2003
- English.
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In: Biochemical and biophysical research communications. - 0006-291X. ; 312:4, s. 1325-34
- Related links:
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https://gup.ub.gu.se...
Abstract
Subject headings
Close
- Scavenger receptor class B type I (SR-BI) is an HDL receptor that mediates selective HDL lipid uptake. Peroxisomes play an important role in lipid metabolism and peroxisomal targeting signal type 1 (PTS1)-containing proteins are translocated to peroxisomes by the peroxisomal targeting import receptor, Pex5p. We have previously identified a PTS1 motif in the intracellular domain of rat SR-BI. Here, we examine the possible interaction between Pex5p and SR-BI. Expression of a Flag-tagged intracellular domain of SR-BI resulted in translocation to the peroxisome as demonstrated by double labeling with anti-Flag IgG and anti-catalase IgG analyzed by confocal microscopy. Immunoprecipitation experiments with anti-SR-BI antibody showed that Pex5p co-precipitated with SR-BI. However, when an antibody against Pex5p was used for immunoprecipitation, only the 57kDa, non-glycosylated form, of SR-BI co-precipitated. We conclude that the PTS1 domain of SR-BI is functional and can mediate peroxisomal interaction via Pex5p, in vitro.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology (hsv//eng)
Keyword
- Amino Acid Sequence
- Animals
- Antigens
- CD36
- chemistry
- metabolism
- Binding Sites
- CHO Cells
- COS Cells
- Cercopithecus aethiops
- Cricetinae
- Cricetulus
- Lipoproteins
- HDL
- metabolism
- Membrane Proteins
- Molecular Biology
- Peroxisomes
- metabolism
- Protein Binding
- Protein Structure
- Tertiary
- Rats
- Receptors
- Cytoplasmic and Nuclear
- chemistry
- metabolism
- Receptors
- Immunologic
- Receptors
- Lipoprotein
- Receptors
- Scavenger
- Scavenger Receptors
- Class B
- Sequence Homology
- Amino Acid
- Structure-Activity Relationship
Publication and Content Type
- ref (subject category)
- art (subject category)
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