Sökning: L773:1531 2267 OR L773:1094 8341
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Comparative transcr...
Comparative transcriptomics of hepatic differentiation of human pluripotent stem cells and adult human liver tissue
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- Ghosheh, Nidal (författare)
- Högskolan i Skövde,Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin,Institute of Biomedicine, Department of Clinical Chemistry and Transfusion Medicine,Forskningscentrum för Systembiologi,Institutionen för biovetenskap,Institute of Biomedicine, Department of Clinical Chemistry and Transfusion Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden,Bioinformatik, Bioinformatics
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- Kuppers-Munther, B. (författare)
- Takara Bio Europe Aktiebolaget, Gothenburg, Sweden
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- Asplund, A. (författare)
- Takara Bio Europe Aktiebolaget, Gothenburg, Sweden
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- Edsbagge, J. (författare)
- Takara Bio Europe Aktiebolaget, Gothenburg, Sweden
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- Ulfenborg, Benjamin (författare)
- Högskolan i Skövde,Institutionen för biovetenskap,Forskningscentrum för Systembiologi,Bioinformatik, Bioinformatics
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- Andersson, T. B. (författare)
- Cardiovascular and Metabolic Diseases, Innovative Medicines and Early Development Biotech Unit, AstraZeneca, Mölndal, Sweden / Department of Physiology and Pharmacology, Section of Pharmacogenetics, Karolinska Institutet, Stockholm, Sweden
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- Bjorquist, P. (författare)
- NovaHep Aktiebolaget, Gothenburg, Sweden
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- Andersson, C. X. (författare)
- Takara Bio Europe Aktiebolaget, Gothenburg, Sweden
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- Carén, Helena, 1979 (författare)
- Gothenburg University,Göteborgs universitet,Sahlgrenska Cancer Center,Institutionen för biomedicin, avdelningen för patologi,Institute of Biomedicine, Department of Pathology,Sahlgrenska Cancer Center, Department of Pathology, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
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- Simonsson, Stina, 1969 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin,Institute of Biomedicine, Department of Clinical Chemistry and Transfusion Medicine,Institute of Biomedicine, Department of Clinical Chemistry and Transfusion Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
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- Sartipy, Peter (författare)
- Högskolan i Skövde,Institutionen för biovetenskap,Forskningscentrum för Systembiologi,AstraZeneca Research and Development, Global Medicines Development Cardiovascular and Metabolic Diseases Global Medicines Development Unit, Mölndal, Sweden,Bioinformatik, Bioinformatics
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- Synnergren, Jane (författare)
- Högskolan i Skövde,Institutionen för biovetenskap,Forskningscentrum för Systembiologi,Bioinformatik, Bioinformatics
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(creator_code:org_t)
- American Physiological Society, 2017
- 2017
- Engelska.
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Ingår i: Physiological Genomics. - : American Physiological Society. - 1094-8341 .- 1531-2267. ; 49:8, s. 430-446
- Relaterad länk:
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https://www.physiolo...
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https://doi.org/10.1...
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https://his.diva-por... (primary) (Raw object)
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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http://kipublication...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- Hepatocytes derived from human pluripotent stem cells (hPSC-HEP) have the potential to replace presently used hepatocyte sources applied in liver disease treatment and models of drug discovery and development. Established hepatocyte differentiation protocols are effective and generate hepatocytes, which recapitulate some key features of their in vivo counterparts. However, generating mature hPSC-HEP remains a challenge. In this study, we applied transcriptomics to investigate the progress of in vitro hepatic differentiation of hPSCs at the developmental stages, definitive endoderm, hepatoblasts, early hPSC-HEP, and mature hPSC-HEP, to identify functional targets that enhance efficient hepatocyte differentiation. Using functional annotation, pathway and protein interaction network analyses, we observed the grouping of differentially expressed genes in specific clusters representing typical developmental stages of hepatic differentiation. In addition, we identified hub proteins and modules that were involved in the cell cycle process at early differentiation stages. We also identified hub proteins that differed in expression levels between hPSC-HEP and the liver tissue controls. Moreover, we identified a module of genes that were expressed at higher levels in the liver tissue samples than in the hPSC-HEP. Considering that hub proteins and modules generally are essential and have important roles in the protein-protein interactions, further investigation of these genes and their regulators may contribute to a better understanding of the differentiation process. This may suggest novel target pathways and molecules for improvement of hPSC-HEP functionality, having the potential to finally bring this technology to a wider use.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
Nyckelord
- human pluripotent stem cell
- stem cell-derived hepatocytes
- liver tissue
- differentiation
- HEPATOCYTE-LIKE CELLS
- GENE-EXPRESSION
- BIOLOGICAL NETWORKS
- MODELS
- METABOLISM
- CHALLENGES
- PATHWAY
- INJURY
- LEVEL
- FETAL
- human pluripotent stem cell
- Bioinformatik
- INF501 Integration of -omics Data
- INF502 Biomarkers
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
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Ghosheh, Nidal
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Kuppers-Munther, ...
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Asplund, A.
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Edsbagge, J.
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Ulfenborg, Benja ...
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Andersson, T. B.
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visa fler...
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Bjorquist, P.
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Andersson, C. X.
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Carén, Helena, 1 ...
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Simonsson, Stina ...
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Sartipy, Peter
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Synnergren, Jane
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visa färre...
- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinska och f ...
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och Cell och molekyl ...
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinsk biotek ...
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och Medicinsk biotek ...
- Artiklar i publikationen
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Physiological Ge ...
- Av lärosätet
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Göteborgs universitet
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Karolinska Institutet
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Högskolan i Skövde