High resolution X-ray structures of the oxidised and reduced forms of nitrite reductase from Rhodobacter sphaeroides 2.4.3

Jacobson, Frida,1975Chalmers tekniska högskola,Chalmers University of Technology (författare)

Nitrite reductase is an enzyme operating in the denitrification pathway which catalyses the conversion of nitrite (NO2(-)) to gaseous nitric oxide (NO). Here, crystal structures of the oxidized and reduced forms of the copper-containing nitrite reductase from Rhodobacter sphaeroides 2.4.3 are presented at 1.74 and 1.85 A resolution, respectively. Whereas the structure of the enzyme is very similar to those of other copper-containing nitrite reductases, folding as a trimer and containing two copper sites per monomer, the structures reported here enable conformational differences between the oxidized and reduced forms of the enzyme to be identified. In the type 1 copper site, a rotational perturbation of the side chain of the copper ligand Met182 occurs upon reduction. At the type 2 copper site, a dual conformation of the catalytic residue His287 is observed in the oxidized structure but is lacking in the reduced structure, such that the interactions of the oxidized type 2 copper ion can be regarded as adopting octahedral geometry. These findings shed light on the structural mechanism of the reduction of a copper-bound nitrite to nitric oxide and water.

NATURVETENSKAP Biologi Biokemi och molekylärbiologi hsv//swe
NATURAL SCIENCES Biological Sciences Biochemistry and Molecular Biology hsv//eng
Copper
chemistry
Crystallography
X-Ray
Hydrogen-Ion Concentration
Nitrite Reductases
chemistry
metabolism
Oxidation-Reduction
Protein Conformation
Rhodobacter sphaeroides
enzymology
Rhodobacter sphaeroides

Guo, KAstraZeneca AB (författare)
Olesen, Kenneth,1966Gothenburg University,Göteborgs universitet,Institutionen för medicinsk och fysiologisk kemi,Institute of Medical Biochemistry,University of Gothenburg(Swepub:gu)xoleke (författare)
Ökvist, Mats,1970Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry,University of Gothenburg (författare)
Neutze, Richard,1969Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)xneuri (författare)
Sjölin, Lennart,1949Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry,University of Gothenburg(Swepub:gu)xsjoll (författare)
Chalmers tekniska högskolaAstraZeneca AB (creator_code:org_t)

Av författaren/redakt...: Jacobson, Frida, ...; Guo, K; Olesen, Kenneth, ...; Ökvist, Mats, 19 ...; Neutze, Richard, ...; Sjölin, Lennart, ...

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