WFRF:(Leach Susannah 1983)
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- Leach, Susannah,1983Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi,Institute of Biomedicine, Department of Microbiology and Immunology (author)
Cross-reactivity and avidity of antibody responses induced in humans by the oral inactivated multivalent enterotoxigenic Escherichia coli (ETEC) vaccine ETVAX
- Article/chapterEnglish2017
Publisher, publication year, extent ...
- Elsevier BV,2017
Numbers
- LIBRIS-ID:oai:gup.ub.gu.se/258164
- https://gup.ub.gu.se/publication/258164URI
- https://doi.org/10.1016/j.vaccine.2017.06.006DOI
Supplementary language notes
- Language:English
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- SwePubSwePub
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Notes
- We investigated whether the oral inactivated, multivalent enterotoxigenic Escherichia coli (ETEC) vaccine ETVAX, consisting of four E. coli strains over-expressing the colonisation factors (CFs) CFA/I, CS3, CS5 and CS6, combined with the toxoid LCTBA, could induce cross-reactive antibodies to CFs related to the CFA/I and CS5 families. We also evaluated the avidity of vaccine induced antibodies against the toxoid and CFs. Cross-reactivity was analysed in mucosal (faecal and antibodies in lymphocyte supernatants, ALS) samples, and antibody avidity in serum and ALS samples, from two phase I trials: a primary vaccination study, where two oral doses of ETVAX were given the double mutant heat labile toxin (dmLT) adjuvant at a 2-week interval, and a booster vaccination study, where a single booster dose of ETVAX was given 13-23 months after primary vaccinations. We found that 65-90% of subjects who had responded to CFA/I in ALS or faecal specimens also developed cross-reactive antibodies to the related CFs tested, i.e. CS1, CS14 and CS17, and that approximately 80% of those responding to CS5 also responded to the closely related CS7. For subjects who had developed cross-reactive antibodies, the magnitudes of responses against vaccine CFs and related non-vaccine CFs were comparable. Using both a simple method of antibody avidity determination based on limiting antigen dilution, as well as a chaotropic ELISA method, we found that the avidity of serum and ALS antibodies to key vaccine antigens increased after a late booster dose compared to after primary vaccination. Our results suggest that the cross-reactive antibody responses against multiple CFs may result in expanded ETEC strain coverage of ETVAX and that repeated vaccinations induce vaccine-specific antibodies with increased binding capacity.
Subject headings and genre
- NATURVETENSKAP Biologi Immunologi hsv//swe
- NATURAL SCIENCES Biological Sciences Immunology hsv//eng
- ETEC vaccine
- Colonisation factors
- Antibodies
- Mucosal IgA
- Cross-reactivity
- Avidity
- factor-antigen-i
- colonization-factor
- immunological memory
- immune-responses
- bangladeshi children
- developing-countries
- diarrheal
- patients
- cell responses
- dmlt adjuvant
- immunogenicity
- Immunology
- Research & Experimental Medicine
Added entries (persons, corporate bodies, meetings, titles ...)
- Lundgren, Anna,1974Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi,Institute of Biomedicine, Department of Microbiology and Immunology(Swepub:gu)xlannj (author)
- Carlin, N. (author)
- Löfstrand, Madeleine,1986Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi,Institute of Biomedicine, Department of Microbiology and Immunology(Swepub:gu)xlofma (author)
- Svennerholm, Ann-Mari,1947Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi,Institute of Biomedicine, Department of Microbiology and Immunology(Swepub:gu)xsannx (author)
- Göteborgs universitetInstitutionen för biomedicin, avdelningen för mikrobiologi och immunologi (creator_code:org_t)
Related titles
- In:Vaccine: Elsevier BV35:32, s. 3966-39730264-410X
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