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  • Sandberg Wranne, Moa,1986Chalmers tekniska högskola,Chalmers University of Technology (author)

Toward Complete Sequence Flexibility of Nucleic Acid Base Analogue FRET

  • Article/chapterEnglish2017

Publisher, publication year, extent ...

  • 2017-06-27
  • American Chemical Society (ACS),2017

Numbers

  • LIBRIS-ID:oai:gup.ub.gu.se/258323
  • https://gup.ub.gu.se/publication/258323URI
  • https://doi.org/10.1021/jacs.7b04517DOI
  • https://research.chalmers.se/publication/251272URI

Supplementary language notes

  • Language:English

Part of subdatabase

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  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • Forster resonance energy transfer (FRET) using fluorescent base analogues is a powerful means of obtaining high-resolution nucleic acid structure and dynamics information that favorably complements techniques such as NMR and X-ray crystallography. Here, we expand the base base FRET repertoire with an adenine analogue FRET-pair. Phosphoramidite-protected quadracyclic 2'-deoxyadenosine analogues qAN1 (donor) and qA(nitro) (acceptor) were synthesized and incorporated into DNA by a genetic, reliable, and high-yielding route, and both constitute excellent adenine analogues. The donor, qAN1, has quantum yields reaching 21% and 11% in single- and double-strands, respectively. To the best of our knowledge, this results in the highest average brightness of an adenine analogue inside DNA. Its potent emissive features overlap well with the absorption of qA(nitro), and thus enable accurate FRET-measurements over more than one turn of B-DNA. As we have shown previously for our cytosine analogue FRET-pair, FRET between qAN1 and qA(nitro) positioned at different base separations inside DNA results in efficiencies that are highly dependent on both distance and orientation. This facilitates significantly enhanced resolution in FRET structure determinations, demonstrated here in a study of conformational changes of DNA upon binding of the minor groove binder netropsin. Finally, we note that the donor and acceptor, of our cytosine FRET-pair, tC degrees and tC(nitro), can be conveniently combined with the acceptor and donor of our current adenine pair, respectively. Consequently, our base analogues can now measure base base FRET between 3 of the 10 possible base combinations and, through base-complementarity, between all sequence positions in a duplex.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

  • Füchtbauer, Anders Foller,1984Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)foller (author)
  • Dumat, Blaise,1984Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)blaise (author)
  • Bood, MattiasGothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,University of Gothenburg,AstraZeneca AB(Swepub:gu)xbooma (author)
  • El-Sagheer, A. H.University Of Oxford,Suez University (author)
  • Brown, T.University Of Oxford (author)
  • Gradén, Henrik,1968AstraZeneca AB(Swepub:cth)grade (author)
  • Grötli, MortenGothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,University of Gothenburg(Swepub:cth)grotli (author)
  • Wilhelmsson, Marcus,1974Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)mawi (author)
  • Chalmers tekniska högskolaInstitutionen för kemi och molekylärbiologi (creator_code:org_t)

Related titles

  • In:Journal of the American Chemical Society: American Chemical Society (ACS)139:27, s. 9271-92800002-78631520-5126

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