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miR-16 and miR-103 ...
miR-16 and miR-103 impact 5-HT4 receptor signalling and correlate with symptom profile in irritable bowel syndrome
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- Wohlfarth, C. (author)
- Heidelberg University
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- Schmitteckert, S. (author)
- Heidelberg University
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- Hartle, J. D. (author)
- Heidelberg University
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- Andreasson, Anna (author)
- Stockholms universitet,Stockholm University,Karolinska Institute,Stressforskningsinstitutet,Karolinska Institutet, Sweden
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- Dweep, H. (author)
- Heidelberg University,National Center for Toxicological Research
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- Fortea, M. (author)
- Vall d'Hebron University Hospital,Autonomous University of Barcelona
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- Assadi, G. (author)
- Karolinska Institutet,Karolinska Institute,Heidelberg University
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- Braun, A. (author)
- Heidelberg University
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- Mederer, T. (author)
- Heidelberg University
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- Pohner, S. (author)
- Karolinska Institutet
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- Becker, P. P. (author)
- Heidelberg University
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- Fischer, C. (author)
- Heidelberg University
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- Granzow, M. (author)
- Heidelberg University
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- Monnikes, H. (author)
- Martin-Luther-Hospital
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- Mayer, E. A. (author)
- University of California, Los Angeles
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- Sayuk, G. (author)
- Washington University in St. Louis
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- Boeckxstaens, G. (author)
- University Hospitals Leuven
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- Wouters, M. M. (author)
- University Hospitals Leuven,University Hospital of Wϋrzburg,Fraunhofer Institute for Interfacial Engineering and Biotechnology
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- Simrén, Magnus, 1966 (author)
- University of Gothenburg,Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för invärtesmedicin och klinisk nutrition,Institute of Medicine, Department of Internal Medicine and Clinical Nutrition
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- Lindberg, G. (author)
- Karolinska Institutet,Karolinska Institute,Karolinska University Hospital
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- Ohlsson, Bodil (author)
- Lund University,Lunds universitet,Internmedicin - epidemiologi,Forskargrupper vid Lunds universitet,Internal Medicine - Epidemiology,Lund University Research Groups,Skåne University Hospital
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- Schmidt, P. T. (author)
- Karolinska Institute,Karolinska University Hospital
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- Dlugosz, A. (author)
- Karolinska Institutet,Karolinska Institute,Karolinska University Hospital,Biodonostia Health Research Institute
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- Agreus, L. (author)
- Karolinska Institutet
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Andreasson, A. (author)
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D'Amato, M. (author)
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- Burwinkel, B. (author)
- University Hospital Heidelberg,German Cancer Research Centre,Heidelberg University
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- Bermejo, J. L. (author)
- Heidelberg University
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- Roth, R. (author)
- Heidelberg University
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- Lasitschka, F. (author)
- Heidelberg University
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- Vicario, M. (author)
- Autonomous University of Barcelona,Vall d'Hebron University Hospital
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Metzger, M. (author)
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- Santos, J. (author)
- Autonomous University of Barcelona,Vall d'Hebron University Hospital
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Rappold, G. A. (author)
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- Martinez, C. (author)
- Autonomous University of Barcelona,Vall d'Hebron University Hospital,Heidelberg University
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- Niesler, B. (author)
- Heidelberg University
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(creator_code:org_t)
- 2017-10-31
- 2017
- English.
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In: Scientific Reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 7
- Related links:
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https://www.nature.c...
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http://dx.doi.org/10... (free)
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https://doi.org/10.1...
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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https://lup.lub.lu.s...
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https://urn.kb.se/re...
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http://kipublication...
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Abstract
Subject headings
Close
- Irritable bowel syndrome (IBS) is a gut-brain disorder involving alterations in intestinal sensitivity and motility. Serotonin 5-HT4 receptors are promising candidates in IBS pathophysiology since they regulate gut motor function and stool consistency, and targeted 5-HT4R selective drug intervention has been proven beneficial in subgroups of patients. We identified a single nucleotide polymorphism (SNP) (rs201253747) c.*61 T > C within the 5-HT4 receptor gene HTR4 to be predominantly present in diarrhoea-IBS patients (IBS-D). It affects a binding site for the miR-16 family and miR-103/miR-107 within the isoforms HTR4b/i and putatively impairs HTR4 expression. Subsequent miRNA-profiling revealed downregulation of miR-16 and miR-103 in the jejunum of IBS-D patients correlating with symptoms. In vitro assays confirmed expression regulation via three 3'UTR binding sites. The novel isoform HTR4b_2 lacking two of the three miRNA binding sites escapes miR-16/103/107 regulation in SNP carriers. We provide the first evidence that HTR4 expression is fine-tuned by miRNAs, and that this regulation is impaired either by the SNP c.*61 T > C or by diminished levels of miR-16 and miR-103 suggesting that HTR4 might be involved in the development of IBS-D.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Endokrinologi och diabetes (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Endocrinology and Diabetes (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Gastroenterologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Gastroenterology and Hepatology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Hälsovetenskap -- Folkhälsovetenskap, global hälsa, socialmedicin och epidemiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Health Sciences -- Public Health, Global Health, Social Medicine and Epidemiology (hsv//eng)
Keyword
- gene-expression
- target site
- ibs
- disorders
- micrornas
- polymorphisms
- transporter
- disease
- biology
- habit
- Science & Technology - Other Topics
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
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Wohlfarth, C.
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Schmitteckert, S ...
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Hartle, J. D.
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Andreasson, Anna
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Dweep, H.
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Fortea, M.
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show more...
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Assadi, G.
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Braun, A.
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Mederer, T.
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Pohner, S.
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Becker, P. P.
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Fischer, C.
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Granzow, M.
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Monnikes, H.
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Mayer, E. A.
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Sayuk, G.
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Boeckxstaens, G.
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Wouters, M. M.
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Simrén, Magnus, ...
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Lindberg, G.
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Ohlsson, Bodil
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Schmidt, P. T.
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Dlugosz, A.
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Agreus, L.
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Andreasson, A.
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D'Amato, M.
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Burwinkel, B.
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Bermejo, J. L.
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Roth, R.
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Lasitschka, F.
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Vicario, M.
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Metzger, M.
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Santos, J.
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Rappold, G. A.
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Martinez, C.
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Niesler, B.
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Endocrinology an ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Gastroenterology ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Health Sciences
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and Public Health Gl ...
- Articles in the publication
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Scientific Repor ...
- By the university
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University of Gothenburg
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Lund University
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Stockholm University
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Karolinska Institutet