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B-cell activation w...
B-cell activation with CD40L or CpG measures the function of B-cell subsets and identifies specific defects in immunodeficient patients
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Marasco, E. (författare)
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Farroni, C. (författare)
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Cascioli, S. (författare)
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Marcellini, V. (författare)
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Scarsella, M. (författare)
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Giorda, E. (författare)
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Piano Mortari, E. (författare)
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Leonardi, L. (författare)
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Scarselli, A. (författare)
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Valentini, D. (författare)
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Cancrini, C. (författare)
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Duse, M. (författare)
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- Grimsholm, Ola, 1979 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för reumatologi och inflammationsforskning,Institute of Medicine, Department of Rheumatology and Inflammation Research
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Carsetti, R. (författare)
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(creator_code:org_t)
- 2016-11-25
- 2017
- Engelska.
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Ingår i: Eur J Immunol. - : Wiley. - 0014-2980. ; 47:1, s. 131-143
- Relaterad länk:
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https://onlinelibrar...
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Around 65% of primary immunodeficiencies are antibody deficiencies. Functional tests are useful tools to study B-cell functions in vitro. However, no accepted guidelines for performing and evaluating functional tests have been issued yet. Here, we report our experience on the study of B-cell functions in infancy and throughout childhood. We show that T-independent stimulation with CpG measures proliferation and differentiation potential of memory B cells. Switched memory B cells respond better than IgM memory B cells. On the other hand, CD40L, a T-dependent stimulus, does not induce plasma cell differentiation, but causes proliferation of naive and memory B cells. During childhood, the production of plasmablasts in response to CpG increases with age mirroring the development of memory B cells. The response to CD40L does not change with age. In patients with selective IgA deficiency (SIgAD), we observed that switched memory B cells are reduced due to the absence of IgA memory B cells. In agreement, IgA plasma cells are not generated in response to CpG. Unexpectedly, B cells from SIgAD patients show a reduced proliferative response to CD40L. Our results demonstrate that functional tests are an important tool to assess the functions of the humoral immune system.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Reumatologi och inflammation (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Rheumatology and Autoimmunity (hsv//eng)
Nyckelord
- Adolescent
- Adult
- Age Factors
- Antigens
- CD40/metabolism
- B-Lymphocyte Subsets/*immunology/*metabolism
- Biomarkers
- CD40 Ligand/*immunology
- Cells
- Cultured
- Child
- Child
- Preschool
- Humans
- Immunoglobulin A/blood/immunology
- Immunoglobulin Class Switching
- Immunoglobulin M/blood/immunology
- Immunologic Deficiency Syndromes/blood/*immunology/*metabolism
- Immunologic Memory
- Immunophenotyping
- Infant
- Lymphocyte Activation/*immunology
- Oligodeoxyribonucleotides/*immunology
- Phenotype
- Protein Binding
- Receptors
- Antigen
- B-Cell/metabolism
- Young Adult
- B cells
- Functional tests
- Immunodeficiency
- Pediatric immunology
- Selective IgA deficiency
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
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Marasco, E.
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Farroni, C.
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Cascioli, S.
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Marcellini, V.
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Scarsella, M.
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Giorda, E.
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visa fler...
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Piano Mortari, E ...
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Leonardi, L.
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Scarselli, A.
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Valentini, D.
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Cancrini, C.
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Duse, M.
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Grimsholm, Ola, ...
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Carsetti, R.
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visa färre...
- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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och Reumatologi och ...
- Artiklar i publikationen
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Eur J Immunol
- Av lärosätet
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Göteborgs universitet